TOP3A Antikörper

Produktnummer ABIN7120017

Dieser Anti-TOP3A Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von TOP3A in WB, ELISA und IP. Geeignet für Human, Maus und Ratte.

Kurzübersicht für TOP3A Antikörper (ABIN7120017)

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TOP3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-TOP3A Antikörper

Verwendungszweck: TOP3A antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: topoisomerase(DNA) III alpha

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IP: 1:200-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu TOP3A

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Andere Bezeichnung: TOP3A

Hintergrund:

Synonyms: DNA topoisomerase 3-alpha|DNA topoisomerase III alpha|TOP3A|TOP3

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.

Molekulargewicht: 100-110 kDa

Gen-ID: 7156

UniProt: Q13472

Pathways: DNA Reparatur