PMS2 Antikörper

Produktnummer ABIN7117603

Dieser Maus Monoklonal Antikörper detektiert spezifisch PMS2 in WB, ELISA und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für PMS2 Antikörper (ABIN7117603)

Target: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PMS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Klon: 4B8

Produktdetails anti-PMS2 Antikörper

Verwendungszweck: PMS2 antibody

Aufreinigung: Protein A+G purification

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: PMS2 postmeiotic segregation increased 2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IHC: 1:50-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu PMS2

Target: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Andere Bezeichnung: PMS2

Hintergrund:

Synonyms: Mismatch repair endonuclease PMS2|DNA mismatch repair protein PMS2|PMS1 protein homolog 2|PMS2|PMSL2

Background: Component of the post-replicative DNA mismatch repair system(MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha(MSH2-MSH6) or MutS beta(MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha(MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Molekulargewicht: 100 kDa

Gen-ID: 5395

UniProt: P54278

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response