MGEA5 Antikörper (AA 430-630)

Produktnummer ABIN7116206

Der Kaninchen Polyklonal Anti-MGEA5 Antikörper wurde für WB, IHC, IP, ELISA und IF validiert. Er ist geeignet, MGEA5 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für MGEA5 Antikörper (AA 430-630) (ABIN7116206)

Target: MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MGEA5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF)

Produktdetails anti-MGEA5 Antikörper

Bindungsspezifität: AA 430-630

Verwendungszweck: OGA antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 430-630aa

Immunogen: meningioma expressed antigen 5(hyaluronidase)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu MGEA5

Target: MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))

Andere Bezeichnung: OGA

Hintergrund:

Synonyms: Protein O-GlcNAcase (OGA)|Beta-N-acetylglucosaminidase|Beta-N-acetylhexosaminidase|Beta-hexosaminidase|Meningioma-expressed antigen 5|N-acetyl-beta-D-glucosaminidase|N-acetyl-beta-glucosaminidase|Nuclear cytoplasmic O-GlcNAcase and acetyltransferase (NCOAT)|OGA|HEXC|KIAA0679|MEA5|MGEA5

Background: Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro)(PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity(PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro), but has about six times lower specific activity than isoform 1.

Molekulargewicht: 130 kDa

Gen-ID: 10724

UniProt: O60502

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process