DDB1 Antikörper (AA 1-400)

Produktnummer ABIN7113313

Dieses Maus Monoklonal-Antikörper erkennt spezifisch DDB1 in WB, IHC, ELISA und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für DDB1 Antikörper (AA 1-400) (ABIN7113313)

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DDB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Klon: 3A0

Produktdetails anti-DDB1 Antikörper

Bindungsspezifität: AA 1-400

Verwendungszweck: DDB1 antibody

Aufreinigung: Protein A+G purification

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-400aa

Immunogen: damage-specific DNA binding protein 1, 127kDa

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IHC: 1:200-1:800

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu DDB1

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Andere Bezeichnung: DDB1

Hintergrund:

Synonyms: DNA damage-binding protein 1|DDB p127 subunit|DNA damage-binding protein a (DDBa)|Damage-specific DNA-binding protein 1|HBV X-associated protein 1 (XAP-1)|UV-damaged DNA-binding factor|UV-damaged DNA-binding protein 1 (UV-DDB 1)|XPE-binding factor (XPE-BF)|Xeroderma pigmentosum group E-complementing protein (XPCe)|DDB1|XAP1

Background: Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex(the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway(the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers(CPD), 6-4 photoproducts(6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX(DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2)(also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8)(the CSA complex) plays a role in transcription-coupled repair(TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.

Molekulargewicht: 127 kDa

Gen-ID: 1642

UniProt: Q16531

Pathways: DNA Reparatur