CPSF3 Antikörper

Produktnummer ABIN7112880

Dieser Anti-CPSF3 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von CPSF3 in WB und ELISA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für CPSF3 Antikörper (ABIN7112880)

Target: CPSF3 (Cleavage and Polyadenylation Specific Factor 3, 73kDa (CPSF3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPSF3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CPSF3 Antikörper

Verwendungszweck: CPSF3 antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: cleavage and polyadenylation specific factor 3, 73kDa

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu CPSF3

Target: CPSF3 (Cleavage and Polyadenylation Specific Factor 3, 73kDa (CPSF3))

Andere Bezeichnung: CPSF3

Hintergrund:

Synonyms: Cleavage and polyadenylation specificity factor subunit 3|Cleavage and polyadenylation specificity factor 73 kDa subunit (CPSF 73 kDa subunit)|mRNA 3'-end-processing endonuclease CPSF-73|CPSF3|CPSF73

Background: Component of the cleavage and polyadenylation specificity factor(CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product(DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop(SL) and 5' phosphate on the downstream cleavage product(DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product(DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.

Molekulargewicht: 77 kDa

Gen-ID: 51692

UniProt: Q9UKF6