RAD1 Antikörper

Produktnummer ABIN7112556

Der Kaninchen Polyklonal anti-RAD1 Antikörper (ABIN7112556) detektiert spezifisch RAD1 in WB, ELISA, IHC und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für RAD1 Antikörper (ABIN7112556)

Target: RAD1 (Cell Cycle Checkpoint Protein RAD1 (RAD1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktdetails anti-RAD1 Antikörper

Verwendungszweck: RAD1 antibody

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: RAD1 homolog(S. pombe)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu RAD1

Target: RAD1 (Cell Cycle Checkpoint Protein RAD1 (RAD1))

Andere Bezeichnung: RAD1

Hintergrund:

Synonyms: Cell cycle checkpoint protein RAD1 (hRAD1)|Rad1-like DNA damage checkpoint protein|RAD1|REC1

Background: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER). The 9-1-1 complex stimulates DNA polymerase beta(POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds, endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths, and DNA ligase I(LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks(DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.

Molekulargewicht: 32 kDa

Gen-ID: 5810

UniProt: O60671

Pathways: M Phase