LIM Domain Kinase 1 Antikörper (AA 459-508)

Produktnummer ABIN7091334

Dieses Maus Monoklonal-Antikörper erkennt spezifisch LIM Domain Kinase 1 in WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für LIM Domain Kinase 1 Antikörper (AA 459-508) (ABIN7091334)

Target: LIM Domain Kinase 1 (LIMK1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LIM Domain Kinase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: L6K5

Produktdetails anti-LIM Domain Kinase 1 Antikörper

Bindungsspezifität: AA 459-508

Homologie: Human,Mouse

Immunogen: KLH conjugated synthetic peptide derived from human LIMK1

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Andere Bezeichnung: LIMK1

Hintergrund:

Synonyms: EC 2.7.11.1, LIM domain kinase 1, LIM motif-containing protein kinase, LIMK, LIMK-1, LIMK2, LIMK-2, LIM domain kinase 2, LIMK1_HUMAN.

Background: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.

Gen-ID: 3984

UniProt: P53667

Pathways: Caspase Kaskade in der Apoptose, Regulation of Cell Size, CXCR4-mediated Signaling Events