Rekombinanter Mesothelin Antikörper

Produktnummer ABIN7072729

Dieser Maus Monoklonal Antikörper detektiert spezifisch Mesothelin in ELISA, FACS und IP. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter Mesothelin Antikörper (ABIN7072729)

Target: Mesothelin (MSLN)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Mesothelin Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Klon: 11-25

Produktdetails anti-Mesothelin Antikörper

Verwendungszweck: Anti-Mesothelin [11-25], Mouse IgG2b, kappa

Spezifität:

This antibody binds the human mesothelin protein, which is highly expressed in many human cancers, including the majority of pancreatic adenocarcinomas, ovarian cancers, and mesotheliomas.

Application Notes original Clone: It is recommended that this antibody be used in combination with mAb IC14-30 to detect mesothelin with high sensitivity in a capture ELISA. This antibody can also immunoprecipitate recombinant mesothelin. NCI-H226 and NCI-H520 were incubated with anti-MSLN antibodies (11-25) or an isotype-matched control antibody and followed by PE-conjugate anti-mouse IgG. Antigen expression was detected by flow cytometry (Iwahori et al., 2008, PMID: 18394747). ⁶⁴Cu labeled 11-25 was used in vitro cell binding assay and in vivo positron emission tomography (PET) imaging in the tumor-bearing mice (Kobayashi et al., 2015, PMID: 25883990).

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with the purified recombinant mesothelin protein.

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2b

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Mesothelin

Target: Mesothelin (MSLN)

Andere Bezeichnung: Mesothelin

Hintergrund: MSLN, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process