Rekombinanter CD33 Antikörper

Produktnummer ABIN7072725

Dieses Anti-CD33-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD33 in FACS, IF und RIA. Geeignet für Human.

Kurzübersicht für Rekombinanter CD33 Antikörper (ABIN7072725)

Target: CD33

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD33 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Radioimmunoassay (RIA)

Klon: M195

Produktdetails anti-CD33 Antikörper

Verwendungszweck: Anti-CD33 [M195], Rabbit IgG, kappa

Spezifität: This antibody binds CD33, which is a 67 kD glycoprotein expressed on monocytes, hematopoetic progenitors, early myeloid progenitor cells and myeloid leukemias (acute and chronic) but not expressed on mature myeloid cells, normal stem cells, other hemtopoietic cells and non hematopoietic human tissues.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with live leukemic myeloblasts.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody is recommended for myeloid differentiation studies, diagnosis of acute non-lymphoid leukemia cells and therapeutic treatment of myelois leukemia. Specificity of this antibody to CD33 was determined using RIA and immunoabsorption. Immunoflurorescence assay showed that the antigen is not expressed on adult human tissues (PMID: 2716349). Flow cytometric analysis was used to confirm reactivity of M195 with fresh hematopoietic neoplasms in the blood or bone marrow. In combination with MY9, this antibody is useful in diagnosis of acute myelogenous leukemia(AML) (PMID: 2725060). Murine antibody M195 was used in the phase I trial of AML (PMID: 1999719). Humanized IgG1 and IgG3 versions of this antibody has higher apparent binding affinity for CD33 antigen than the chimeric and parent murine version (PMID: 1737932, 1458463). The increase in binding affinity resulted from eliminating an N-linked glycosylation site at residue 73 in the heavy chain variable region in the course of humanization (PMID: 8232322). Preliminary trials to treat AML with humanized antibody showed specific bone marrow targeting without an immunogenic response (PMID: 8124221).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD33

Target: CD33

Andere Bezeichnung: CD33

Hintergrund: Gp67, Siglec-3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3

UniProt: P20138