Rekombinanter Cytokeratin 7 Antikörper

Produktnummer ABIN7072675

Produktdetails anti-Cytokeratin 7 Antikörper

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser Cytokeratin 7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: Anti-Cytokeratin 7 [OV-TL 12/30], Rabbit IgG, kappa

Spezifität: This antibody recognizes cytokeratin-7. Cytokeratins are a subfamily of intermediate filament proteins but this antibody does not recognize other intermediate filament proteins. The antibody reacts with proteins that are found in most ductal, glandular, transitional, and biliary duct epithelial cells.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was originally raised by immunizing BALB/c mice with ovarian carcinoma cell line OTN11.

Klon: OV-TL 12-30

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry. Cytokeratin 7 (CK7) labeling can help distinguish between lung, breast carcinomas, and urothelial carcinomas that typically stain positive, and colon and prostate carcinomas that typically lack CK7 expression (Jerome et al., 2004, Logani et al., 2003, Murray et al., 2004, Roy et al., 2011). CK7 is a common marker of primary lung adenocarcinomas (almost all cases) with a lower specificity since it is also observed in other primary lung carcinomas and non-pulmonary carcinomas (Jerome et al., 2004). Anti-cytokeratin 7 has also been useful in the differential diagnosis of ovarian neoplasms (McCluggage et al., 2005). Furthermore, this antibody is a marker for distinguishing between the conjunctival and corneal surface epithelia which may be used in the diagnosis of limbal stem cell deficiency (LSCD) (Jirsova et al., 2011, pmid: 21693612). Finally, OV-TL 12/30 has proven very effective and popular in immunohistochemistry, for instance, one group used it to label cytokeratin-7 in human term placental samples (König et al., 2014, pmid: 24326460).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Andere Bezeichnung: Cytokeratin 7 (KRT7 Produkte)

Hintergrund: CK-7, Keratin, type II cytoskeletal 7, Cytokeratin-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, CK7, CK 7

UniProt: P08729