Rekombinanter Transferrin Receptor Antikörper

Produktnummer ABIN7072543

Dieser Ratte Monoklonal Antikörper detektiert spezifisch Transferrin Receptor in FACS und Dep. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für Rekombinanter Transferrin Receptor Antikörper (ABIN7072543)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Receptor Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Depletion (Dep)

Klon: RI7 217-1-3

Produktdetails anti-Transferrin Receptor Antikörper

Verwendungszweck: Anti-Transferrin receptor [RI7 217.1.3], Rat IgG2a, kappa

Spezifität:

The transferrin receptor is necessary for cellular iron uptake by the process of receptor-mediated endocytosis. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis.

Application Notes original Clone: This antibody was generated and used in a flow cytometry assay to identify murine hematopoietic progenitor cells expressing the transferrin receptor (Lesley et al., 1984, PMID: 6319033). The original format of this antibody was tested and found to moderately reduce cell growth. A Fab version of this antibody was generated, its binding was tested, and it was compared to its full antibody counterpart. In contrast to the original antibody format, the Fab fragment did not cause receptor down-regulation or accelerated degradation. Both the Fab fragment and the full antibody were successfully used in an IF staining assay (Lesley et al., 1989, PMID: 2653853). The original fotmat of this antibody was demonstrated to undergo receptor-mediated transcytosis across the blood-brain barrier (BBB), as confirmed by capillary depletion studies, and to effectively transport into the mouse brain in vivo (Lee et al., 2000, PMID: 10688622). Injection of this antibody into mice resulted in CD71⁺ erythroid splenocyte cell depletion (Torow et al., 2015, PMID: 26195040).

Aufreinigung: Protein A affinity purified

Immunogen: The antibody was obtained from a fusion of the murine myeloma S 194/5.XXO.BU. 1 with spleen cells from a BDIX rat immunized with the Friend erythroleukemia 745.6.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Rat

OriginalFormat: IgG2a

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Andere Bezeichnung: Transferrin receptor

Hintergrund: CD71, Transferrin receptor protein 1, TR, , TfR1, Trfr, R17 217.1.3, RI7-217

Pathways: Transition Metal Ion Homeostasis