Rekombinanter TIM3 Antikörper

Produktnummer ABIN7072498

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch TIM3 in FACS, IHC, IF und BP. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für Rekombinanter TIM3 Antikörper (ABIN7072498)

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TIM3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Blocking Peptide (BP)

Klon: 2C12

Produktdetails anti-TIM3 Antikörper

Verwendungszweck: Anti-Tim-3 [2C12], Rat IgG1, kappa

Spezifität: This antibody is specific for murine TIM-3, a Th1-specific cell surface protein. TIM-3 is a type I transmembrane protein and contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. This antibody binds to the BALB/c allele of TIM-3 while reactivity to the C57Bl/6 allele is significantly weaker.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising rats with TIM-3:Ig fusion protein emulsified in complete Freund's adjuvant.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: This antibody was used in FACS, immunohistochemistry, immunofluorescence and a murine experimental autoimmune encephalomyelitis model to study the effects of TIM-3 triggering in dendritic cells (Anderson et al., 2007, PMID: 18006747). Furthermore, this antibody has been used in multiple FACS analyses for diverse immuno-oncological applications, such as to delineate how TIM-3 signaling on cells of the innate immune system critically influences regulation of the adaptive immune response (Frisancho-Kiss et al., 2006), to demonstrate the reversal of T cell exhaustion and restoration of anti-tumor immunity upon co-targeting Tim-3 and PD-1 pathways (Sakuishi et al., 2010, PMID: 20819927), and to examine the therapeutic potential of dendritic cell based vaccines against malignant glioma (Dey et al., 2015). This antibody has also been used in immunohistochemistry to aid the elucidation of the effects of Th2 immune deviation on corneal allograft survival and possible mechanisms of graft rejection (Beauregard et al., 2005). In addition, employing this anti-TIM-3 antibody as part of the combination immuntherapy (anti-TIM3 + anti-PD-1 +/- anti-CTLA-4) has been shown to be effective against experimental and carcinogen-induced tumors in mice (Sakuishi et al., 2010, PMID: 20819927) (Ngiow et al., 2011, PMID: 21430066).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu TIM3

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Andere Bezeichnung: Tim-3

Substanzklasse: Virus

Hintergrund: CD366, HAVcr-2, HAVCR2, Hepatitis A virus cellular receptor 2 homolog, T-cell immunoglobulin and mucin domain containing 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIMD-3, TIM3, 8B.2C12

UniProt: Q8VIM0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints