Rekombinanter CD70 Antikörper

Produktnummer ABIN7072496

Der Ratte Monoklonal Anti-CD70-Antikörper wurde für WB, FACS, IF und BP validiert. Er ist geeignet, CD70 in Proben von Maus zu detektieren.

Kurzübersicht für Rekombinanter CD70 Antikörper (ABIN7072496)

Target: CD70 (CD70 Molecule (CD70))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Blocking Peptide (BP)

Klon: TAN1-6

Produktdetails anti-CD70 Antikörper

Verwendungszweck: Anti-CD70 [TAN1-6], Rat IgG2a, kappa

Spezifität: This antibody is specific for murine CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising LOU rats with soluble recombinant CD70:Fc fusion protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: This antibody has been used in various FACS analyses to reveal a critical role for CD70 in CD8 T cell priming by CD40-licensed antigen-presenting cells (Taraban et al, 2004), to demonstrate the differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells (Bak et al, 2012), to examine how temporal changes in dendritic cell subsets, cross-priming and costimulation via CD70 control CD8(+) T cell responses to influenza (Ballesteros-Tato et al, 2010), and to show that CD70 is downregulated by interaction with CD27 (Kuka et al, 2013). In addition, this antibody has been used in vivo to block the CD70-CD27 interaction as part of the two studies examining how CD70 is critically required for the priming of CD8+ T cells (Taraban et al, 2004, Taraban et al, 2006).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD70

Target: CD70 (CD70 Molecule (CD70))

Andere Bezeichnung: CD70

Hintergrund: TNFSF7, CD27 ligand, CD27-L, Cd27l, CD27LG, CD70 antigen, Ki-24 antigen, Tnfsf7, tumor necrosis factor (ligand) superfamily, member 7, Tumor necrosis factor ligand superfamily member 7

UniProt: O55237

Pathways: Cancer Immune Checkpoints