Rekombinanter CCRL2 Antikörper (AA 1-24)

Produktnummer ABIN7072484

Der Ratte Monoklonal Anti-CCRL2-Antikörper wurde für WB, ELISA, IHC, FACS, ICC, Func und IP validiert. Er ist geeignet, CCRL2 in Proben von Maus zu detektieren.

Kurzübersicht für Rekombinanter CCRL2 Antikörper (AA 1-24) (ABIN7072484)

Target: CCRL2 (Chemokine (C-C Motif) Receptor-Like 2 (CCRL2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CCRL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Functional Studies (Func), Immunoprecipitation (IP)

Klon: BZ5B8

Produktdetails anti-CCRL2 Antikörper

Bindungsspezifität: AA 1-24

Verwendungszweck: Anti-CCRL2 [BZ5B8], Rat IgG2a, kappa

Spezifität: This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2 presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2 antibody binding.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: This antibody has been used in various FACS analyses, for instance, to confirm that it is only specific to mCCRL2-hemagglutinin (HA)/L1.2 transfectants and that peritoneal macrophages treated with LPS, TNFα, IFN-γ, or poly:IC up-regulate mCCRL2 protein expression (Zabel et al., 2008). Of note, this antibody binding to mouse peritoneal mast cells could be inhibited by chemerin, a protein ligand for signaling receptor CMKLR1 (Zabel et al., 2008). This anti-CCRL2 antibody can be used in wide variety of assays to study leukocytes and immune response. For example, this antibody has been used to examine the expression, regulation, and function of atypical chemerin receptor CCRL2 on endothelial cells (Monnier et al., 2012) and to delineate how the chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses (Pachynski et al., 2012).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CCRL2

Target: CCRL2 (Chemokine (C-C Motif) Receptor-Like 2 (CCRL2))

Andere Bezeichnung: CCRL2

Hintergrund: CCR11, C-C chemokine receptor-like 2, Chemokine receptor CCR11, CKRX, CRAM-A, CRAM-B, G-protein coupled beta chemokine receptor, L-CCR, Lipopolysaccharide-inducible C-C chemokine receptor

UniProt: O35457