Rekombinanter CD80 Antikörper

Produktnummer ABIN7072473

Dieses Anti-CD80-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD80 in FACS, WB, IHC, Func und BP. Geeignet für Maus.

Kurzübersicht für Rekombinanter CD80 Antikörper (ABIN7072473)

Target: CD80

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD80 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Functional Studies (Func), Blocking Peptide (BP)

Klon: RM80

Produktdetails anti-CD80 Antikörper

Verwendungszweck: Anti-CD80 [RM80], Rabbit IgG, kappa

Spezifität: This antibody is specific for murine CD80, a ~55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to the activation of T and B cells, respectively.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody has been used in multiple FACS experiments for various immunological applications, such as to highlight the robustness of the T cell priming process by activated Nlrc5-deficient dendritic cells (Rota et al, 2016), to demonstrate that allogeneic IgG combined with dendritic cell stimuli could induce anti-tumor T cell immunity (Carmi et al, 2015), to confirm the role of aquaporin-9-expressing neutrophils for the development of contact hypersensitivity (Moniaga et al, 2015), and to support the therapeutic potential of targeting B-cell Syk signaling in chronic graft-versus-host disease (Flynn et al, 2015). This antibody has also been used in Western Blot to study the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1-associated cytokines (Yue et al, 2015), as well as in in vitro blocking experiments to confirm the role of CD28 signals for IL-2-mediated survival and proliferation of both CD27(+) and CD27(-) γδ T cell subsets (Ribot et al, 2012). Furthermore, this antibody has been used, together with the anti-CD86 antibody, in numerous in vivo studies, such as in a NZB/W F1 mouse lupus model to clarify the role of CD80 and CD86 in murine Ab-mediated autoimmunity (Nakajima et al, 1995), in graft bearing recipient BALB/c mice to evaluate the roles of CD80 and CD86 costimulatory molecules in corneal allograft rejection (Kagaya et al, 2002), and in transgenic BALB/c mice to demonstrate the critical contribution of CD80 and CD86 in anterior chamber-associated immune deviation (Tsukahara et al, 2005).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD80

Target: CD80

Andere Bezeichnung: CD80

Hintergrund: B7-1, Activation B7-1 antigen, B7, B7 protein, B7-1 protein, B7.1, Cd28l, CD28LG, CD28LG1, LAB7, Ly-53, Ly53, MIC17, T-cell co-stimulatory protein B7-1, T-lymphocyte activation antigen CD80, TS/A-1

UniProt: Q00609

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints