Rekombinanter DARC Antikörper (N-Term)

Produktnummer ABIN7072437

Der Kaninchen Chimeric anti-DARC Antikörper (Klon 2C3) (ABIN7072437) detektiert spezifisch DARC in WB, ELISA, FACS, BP und Inh. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter DARC Antikörper (N-Term) (ABIN7072437)

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser DARC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Blocking Peptide (BP), Inhibition (Inh)

Klon: 2C3

Produktdetails anti-DARC Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-DARC [2C3], Rabbit IgG, kappa

Spezifität:

The antibody specifically binds to the pentapeptide FEDVW of the Duffy Antigen Receptor for Chemokines (DARC). The epitope is located on the N-terminal region on the RBC surface membrane. DARC is a glycoprotein chemokine receptor and the only known receptor for Plasmodium vivax.

Application Notes original Clone: The original antibody (mouse IgG1) was generated and used successfully in ELISA, FC, and inhibition assays, blocking DARC and IL-8 binding. The antibody's epitope was also characterized (Wasniowska et al., 2002, PMID: 12164140). This antibody was successfully used in a WB (Czerwinski et al., 2007 (PMID: 17382291), Smolarek et al., 2015 (PMID: 25706384)). This antibody was successfully used to inhibit P. Vivax invasion (Russell et al., 2011 (PMID: 21768300), Urusova et al., 2019 (PMID: 31133752)).

Produktmerkmale: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Aufreinigung: Protein A affinity purified

Immunogen: CHO cells transfected with cDNA encoding the Duffy glycoprotein.

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu DARC

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Andere Bezeichnung: DARC

Hintergrund: Fy6, Duffy Antigen Receptor for Chemokines, NaM185-2C3

UniProt: Q4VBN9