Rekombinanter GM2 Ganglioside Antikörper

Produktnummer ABIN7072396

Dieser Kaninchen Chimeric Antikörper detektiert spezifisch GM2 Ganglioside in ELISA, IHC, Dep und TLC Overlay. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter GM2 Ganglioside Antikörper (ABIN7072396)

Target: GM2 Ganglioside

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Depletion (Dep), Thin Layer Chromatography Overlay (TLC Overlay)

Klon: L55-81

Produktdetails

Verwendungszweck: Anti-GM2 ganglioside [L55-81], Rabbit IgG, kappa

Spezifität: L55-81 binds specifically to GM2 ganglioside, this antibody shows no reactivity towards GM1, GM3, GD2 or normal tissues- the L55-81 epitope contains both a GalNAc and sialic acid bound to lactose. Ganglioside GM2 (GalNAcβ1-4(NeuNAcα2-3)Galβ1-4Glcβ-1ceramide) is a glycosphingolipid present on the cell surface and a major tumor-differentiation antigen in human melanoma and hepatoma.

Produktmerkmale:

Original Species of Ab: Human

Original Format of Ab: IgM

Aufreinigung: Protein A affinity purified

Immunogen: L55-81 was prepared by isolating EBV-immortalized peripheral blood B lymphocytes from a human donor with melanoma.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: L55-81 reacts positively to melanoma cell lines with high GM2 expression (M14, M24), and shows no binding to normal cell types or cell lines M15 or M25 which have low GM2 expression and high GM3 expression. This specificity can be confirmed by ELISA and TLC immunostaining by testing a variety of gangliosides and neutral glycolipids. L55-81 is able to lyse cells from the M14 melanoma cell line (which has high GM2 expression) in the presence of human or rabbit complement, but not in the absence of complement - this was shown by a [51Cr] release assay. Biotinylated L55-81 has been used for IHC of acetone-fixed sections of tumor tissue in an avidin-biotin complex immunoperoxidase system. Statistically significant positive staining (determined by Fisher's exact test) was displayed with melanoma, colon carcinoma and ovary carcinoma tumor tissues. Staining has also be observed with breast carcinoma, prostate carcinoma and kidney carcinoma.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Antigendetails

Target: GM2 Ganglioside