Rekombinanter Rhodopsin Antikörper (C-Term)

Produktnummer ABIN7072290

Der Kaninchen Monoklonal anti-Rhodopsin Antikörper (Klon Rho 1D4) (ABIN7072290) detektiert spezifisch Rhodopsin in WB, IHC, ELISA, IP, IF und Lbl. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Rind (Kuh), Zebrafisch (Danio rerio) und Amphibien.

Kurzübersicht für Rekombinanter Rhodopsin Antikörper (C-Term) (ABIN7072290)

Target: Rhodopsin (RHO)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Zebrafisch (Danio rerio), Amphibien

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Rhodopsin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Labeling (Lbl)

Klon: Rho 1D4

Produktdetails anti-Rhodopsin Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-Rhodopsin [Rho 1D4], Rabbit IgG, kappa

Spezifität:

The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.

Application Notes original Clone: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.

Kreuzreaktivität: Amphibien, Rind (Kuh), Human, Ratte, Zebrafisch (Danio rerio)

Aufreinigung: Protein A affinity purified

Immunogen: Bleached bovine rod outer segment (ROS).

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Rhodopsin

Target: Rhodopsin (RHO)

Andere Bezeichnung: Rhodopsin

Substanzklasse: Chemical

Hintergrund: Rho-tag, Visual Purple

UniProt: P08100

Pathways: WNT Signalweg, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction