Rekombinanter Rhodopsin Antikörper

Produktnummer ABIN7072289

Der Maus Chimeric Anti-Rhodopsin-Antikörper wurde für WB, IHC, ELISA, IP, IF und Lbl validiert. Er ist geeignet, Rhodopsin in Proben von Human, Rind (Kuh), Ratte, Maus, Zebrafisch (Danio rerio) und Amphibien zu detektieren.

Kurzübersicht für Rekombinanter Rhodopsin Antikörper (ABIN7072289)

Target: Rhodopsin (RHO)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Rind (Kuh), Ratte, Maus, Zebrafisch (Danio rerio), Amphibien

Wirt: Maus

Klonalität: Chimeric

Konjugat: Dieser Rhodopsin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Labeling (Lbl)

Klon: Rho 1D4

Produktdetails anti-Rhodopsin Antikörper

Verwendungszweck: Anti-Rhodopsin [Rho 1D4], Mouse IgG1, kappa

Spezifität: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.

Kreuzreaktivität: Amphibien, Rind (Kuh), Human, Ratte, Zebrafisch (Danio rerio)

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: Bleached bovine rod outer segment (ROS).

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.

Kommentare:

This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Rhodopsin

Target: Rhodopsin (RHO)

Andere Bezeichnung: Rhodopsin

Substanzklasse: Chemical

Hintergrund: Rho-tag, Visual Purple

UniProt: P08100

Pathways: WNT Signalweg, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction