Rekombinanter IL-2 Antikörper

Produktnummer ABIN7072227

Dieses Anti-IL-2-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von IL-2 in WB, ELISA, FACS, IF, Crys und LA. Geeignet für Human.

Kurzübersicht für Rekombinanter IL-2 Antikörper (ABIN7072227)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser IL-2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Crystallization (Crys), Latex Agglutination (LA)

Klon: LNKB-2

Produktdetails anti-IL-2 Antikörper

Verwendungszweck: Anti-IL-2 [LNKB-2], Rabbit IgG, kappa

Spezifität: This antibody binds human IL-2 at the 59-72 site of the IL-2 sequence.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody was quantified by ELISA and used for latex-agglutination analysis of human IL-2. It was measured to bind human IL-2 with high affinity (Ka ~ 3 x 10⁸ M^-1) (Lunev et al., 1990, PMID: 1718463). This antibody has been used successfully for WB and FACS (Stratieva-Taneeva et al., 1993, PMID: 8359822). The crystal structure characterization of the Fab version of this antibody was performed (Fokin et al., 2000, PMID: 11040993). Further crystallization of the Fab version of this antibody with an antigenic peptide revealed that the antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the α-helical character of the epitope fragment (Afonin et al., 2001, PMID: 11468348).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Andere Bezeichnung: IL-2

Hintergrund: Interleukin 2, LNKB2

UniProt: P60568

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation