TRPV5 Antikörper (Intracellular)

Produktnummer ABIN7043855

Der Kaninchen Polyklonal Anti-TRPV5-Antikörper wurde für WB, IHC, IF und IC validiert. Er ist geeignet, TRPV5 in Proben von Human zu detektieren.

Kurzübersicht für TRPV5 Antikörper (Intracellular) (ABIN7043855)

Target: TRPV5 (Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRPV5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-TRPV5 Antikörper

Bindungsspezifität: AA 715-729, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to TRPV5 Channel

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: rabbit - 13,Rat - 11,15 amino acid residues identical

Produktmerkmale: Anti-TRPV5 Antibody is directed against an epitope of human TRPV5. Anti-TRPV5 Antibody (ABIN7043855, ABIN7043996 and ABIN7043997) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize TRPV5 from human, mouse and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GLNLSEGDGEEVYHF, corresponding to amino acid residues 715-729 of human TRPV5

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: ICC|IFC

Negative Control: (ABIN7237501)

Blocking Peptide: (ABIN7237501)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu TRPV5

Target: TRPV5 (Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5))

Andere Bezeichnung: TRPV5

Hintergrund: Transient receptor potential cation channel subfamily V member 5, Calcium transport protein 2, CaT2, Epithelial calcium channel 1, ECaC1, OTRPC3,TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR, the TRP family is comprised of three main subfamilies on the basis of sequence homology, TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family, the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5TRPV5 (ECaC) and TRPV6 (CaT1) are unique members of the TRP family. Both are epithelial Ca2+ channels, form constitutively open channels, share high degree of homology (about 66 % ) with differences in the N- and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV5 and TRPV6 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption.6,7 TRPV5 is preferentially expressed in the kidney.

Alternative names: TRPV5, Transient receptor potential cation channel subfamily V member 5, Calcium transport protein 2, CaT2, Epithelial calcium channel 1, ECaC1, OTRPC3

Gen-ID: 56302

NCBI Accession: NM_019841

UniProt: Q8NDW5