SLC8A1 Antikörper (AA 308-320)

Produktnummer ABIN7043754

Der Kaninchen Polyklonal anti-SLC8A1 Antikörper (ABIN7043754) detektiert spezifisch SLC8A1 in WB, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für SLC8A1 Antikörper (AA 308-320) (ABIN7043754)

Target: SLC8A1 (Solute Carrier Family 8 (Sodium/calcium Exchanger), Member 1 (SLC8A1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC8A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-SLC8A1 Antikörper

Bindungsspezifität: AA 308-320

Verwendungszweck: A Rabbit Polyclonal Antibody to Na+/Ca2+ Exchanger 1 (SLC8A1)

Spezifität: 3rd intracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human,mouse - identical

Produktmerkmale: Anti-NCX1 (SLC8A1) Antibody (ABIN7043754, ABIN7044875 and ABIN7044876) is a highly specific antibody directed against an epitope of the rat Na+/Ca2+ exchange protein 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NCX1 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EVDERDQDDEEAR, corresponding to amino acid residues 308-320 of rat NCX1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IP|IHC|ICC

Negative Control: (ABIN7237196)

Blocking Peptide: (ABIN7237196)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu SLC8A1

Target: SLC8A1 (Solute Carrier Family 8 (Sodium/calcium Exchanger), Member 1 (SLC8A1))

Andere Bezeichnung: SLC8A1

Hintergrund: Sodium/calcium exchanger 1, Na+/Ca2+ exchange protein 1,Ca2+ has proven to be a universal signaling molecule in excitable and non-excitable cells. However, being that its intracellular concentration is 1000 times lower than that of the extracellular milieu, it is important for the cell to keep this ratio for proper function. NCX, a Na+/Ca2+ exchanger is responsible for most of the efflux of Ca2+ from the cell1-3. The NCX transporter is a member of the SLC8 family of solute carriers which in turn belong to the CaCA superfamily1,4,5.NCX-1 is one of three Na+/Ca2+ exchangers (NCX-1, NCX-2, NCX-3) leading to one Ca2+ movement across the plasma membrane in exchange of three Na+ influx. However, the transporter can reverse the direction of the transport if the concentrations of Na+ and Ca2+ change6. The transporter has nine transmembrane domains and intracellular N- and C-terminals. Between tansmembrane domains 5 and 6, the presence of an extra-long intracellular loop, termed the f-loop, is responsible for regulating the activity of NCX-1 via several different mechanisms like ion binding, phosphorylation, etc. The f-loop also has sites which undergo alternative splicing7.Of the three NCX expressed in mammalian cells, NCX-1 is the most widely expressed. Its expression is detected in the heart, brain, and kidney. NCX-1 undergoes alternative splicing in a tissue-dependent manner. The first splice region does not change the overall structure of the protein but rather enables the expression of the gene specific to the tissues which require the expression of the gene. The second splicing site leads to a number of proteins varying in length. NCX-2 expression is much more limited, it is expressed only in neurons. NCX-3 is expressed in skeletal muscle and in some regions of the brain and undergoes alternative splicing in a similar fashion to that of NCX-11,8.Due to its central role in modulating Ca2+ levels in the cell, NCX-1 has become a pharmaceutical target in the development of drugs for various heart diseases and neurological disorders1.

Alternative names: NCX1 (SLC8A1), Sodium/calcium exchanger 1, Na+/Ca2+ exchange protein 1

Gen-ID: 29715

NCBI Accession: NM_021097

UniProt: Q01728

Pathways: Myometrial Relaxation and Contraction