SLC39A8 Antikörper (Extracellular) (FITC)

Produktnummer ABIN7043727

Produktdetails anti-SLC39A8 Antikörper (FITC)

Target: SLC39A8 (Solute Carrier Family 39 (Zinc Transporter), Member 8 (SLC39A8))

Bindungsspezifität: AA 221-234, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC39A8 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Verwendungszweck: A Rabbit Polyclonal Antibody to Zinc Transporter ZIP8 Conjugated to the Fluorescent Dye FITC

Spezifität: 2nd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, Human - 12,14 amino acid residues identical

Produktmerkmale: Anti-ZIP8 (SLC39A8) (extracellular) Antibody (ABIN7043726, ABIN7045350 and ABIN7045351) is a highly specific antibody directed against an epitope of the rat Zinc transporter ZIP8. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry. It has been designed to recognize ZIP8 from human, rat, and mouse samples. \nAnti-ZIP8 (SLC39A8) (extracellular)-FITC Antibody (ABIN7043726, ABIN7045350 and ABIN7045351)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KTYGQNDHTHFRND, corresponding to amino acid residues 221-234 of rat ZIP8

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Kommentare:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7237102)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu SLC39A8

Target: SLC39A8 (Solute Carrier Family 39 (Zinc Transporter), Member 8 (SLC39A8))

Andere Bezeichnung: SLC39A8 (SLC39A8 Produkte)

Hintergrund: Zinc transporter ZIP8, Zrt- and Irt-like protein 8, Solute carrier family 39 member 8, BIGM103,Zinc uptake transporter, ZIP8, belongs to the SLC39 transporter family. ZIP transporters are responsible for zinc transport into the cytoplasm across cellular membranes, either influx from the extracellular space or efflux from intracellular organelles. Regulation and maintenance of proper concentration and distribution of cellular zinc nutrient by ZIP transporters are essential to the function, metabolism, growth, proliferation and survival of cells1,2.ZIP structure contains eight predicted transmembrane (TM) domains while the N- and C-termini are extracellular. A long loop region is present between TM3 and TM4 domains, harboring a histidine-rich region. TM4 and TM5 domains are amphipathic and are thought to form a cavity through which metals are transported1.ZIP8 protein is encoded by the SLC39A8 gene and is highly expressed in T cells. ZIP8 also plays an important role at the onset of inflammation2.Zinc deficiency can cause growth retardation, cognitive impairment, metabolic disorders, infertility, dysfunction of the immune system, abnormalities in T-cells, natural killer cells and monocytes, and reduction in antibody formation1,2. Studies exploring knockdown of ZIP8 protein show a reduction of cellular zinc content, impaired mitochondrial function in response to TNF-α and increased cell death2.

Alternative names: ZIP8 (SLC39A8), Zinc transporter ZIP8, Zrt- and Irt-like protein 8, Solute carrier family 39 member 8, BIGM103

Gen-ID: 295455

NCBI Accession: NM_022154

UniProt: Q5FVQ0