SLC12A1 Antikörper (Intracellular)

Produktnummer ABIN7043674

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SLC12A1 in WB. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für SLC12A1 Antikörper (Intracellular) (ABIN7043674)

Target: SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC12A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SLC12A1 Antikörper

Bindungsspezifität: AA 776-790, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to NKCC2 Transporter

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 13,15 amino acid residues identical

Produktmerkmale: Anti-NKCC2 (SLC12A1) (extracellular) Antibody is directed against an epitope of rat NKCC2 transporter. Anti-NKCC2 (SLC12A1) (extracellular) Antibody (ABIN7043674, ABIN7044815 and ABIN7044816). The antibody recognizes an extracellular epitope and is thus ideal for detecting NKCC2 in living cells. It has been designed to recognize NKCC2 from rat, human and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GYKKNWRKAPLSELE, corresponding to amino acid residues 776-790 of rat NKCC2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7236937)

Blocking Peptide: (ABIN7236937)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu SLC12A1

Target: SLC12A1 (Solute Carrier Family 12 (Sodium/potassium/chloride Transporters), Member 1 (SLC12A1))

Andere Bezeichnung: SLC12A1

Hintergrund: Na+-K+-2Cl- Cotransporter 2, Kidney-specific Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC1, Solute carrier family 12 member 1,Na+-K+-2Cl- cotransporter (NKCC2) belongs to the family of integral plasma membrane proteins, solute carrier family 12 ,the electrically silent, cation-coupled chloride co-transporter family that mediates electroneutral symport of Na+, K+, and Cl- with a stochiometry of 1:1:2, respectively. NKCC2 structure contains a central hydrophobic domain made up of 12 putative transmembrane spanning regions and a long hydrophilic loop connects transmembrane segments 7 and 8 and contains two glycosylation sites. The hydrophobic domain is flanked by a short amino- and a long carboxyl-terminal domain that are located within the cell.NKCC2 encoded by the Slc12a1 gene shows at least six alternatively spliced variants and has the ability to form dimmers. NKCC2 activity and expression can be found in the medullary and cortical thick ascending limb of Henle's loop is the nephron and in macula densa cells.NKCC2 allows Na+ and Cl- entry from the tubule lumen into the thick ascending limb of Henle's loop, a process that allows reabsorbing 25-30 % of the NaCl filtered by the glomeruli. In addition, NKCC2 can sense luminal Cl- concentration and initiates the signal for tubuloglomerular feedback in the specialized cells of the macula densa. Thus, NKCC2 activity is essential for salt conservation and for acute and chronic regulation of water balance.Mutations in the human NKCC2 gene causes Bartter syndrome type 1 characterized by severe natriuresis hypercalciuria and magnesuria, the inability to concentrate urine, leading to hyponatremia, hypochloremia, metabolic alkalosis, and low blood pressure. Opposite to Bartter's syndrome, enhanced activity of NKCC2 NaCl is connected with human and animal hypertension.

Alternative names: NKCC2 (SLC12A1), Na+-K+-2Cl- Cotransporter 2, Kidney-specific Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC1, Solute carrier family 12 member 1

Gen-ID: 25065

NCBI Accession: NM_000338

UniProt: P55016