SLC1A5 Antikörper (2nd Extracellular Loop)

Produktnummer ABIN7886779

Der Kaninchen Polyklonal anti-SLC1A5 Antikörper wird verwendet zum Nachweis von SLC1A5 in Proben von Human. Er wurde validiert für WB, FACS und LCI.

Kurzübersicht für SLC1A5 Antikörper (2nd Extracellular Loop) (ABIN7886779)

Target: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC1A5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-SLC1A5 Antikörper

Bindungsspezifität: 2nd Extracellular Loop, AA 202-217

Verwendungszweck: A Rabbit Polyclonal Antibody to ASCT2 Transporter

Kreuzreaktivität (Details): The antibody will not recognize rat or mouse ASCT2.

Homologie: Human only

Produktmerkmale: Anti-Human ASCT2/SLC1A5 (extracellular) Antibody (ABIN7043669, ABIN7044825 and ABIN7044826) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize SLC1A5 from human samples only. The antibody will not recognize rat or mouse ASCT2.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RSYSTTYEERNITGTR, corresponding to amino acid residues 202 - 217 of human ASCT2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu SLC1A5

Target: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Andere Bezeichnung: SLC1A5

Hintergrund:

Synonyms: Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2

Description: ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways. It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.

Gen-ID: 6510

NCBI Accession: NM_001145144

UniProt: Q15758

Pathways: Dicarboxylic Acid Transport, Warburg Effekt