SLC1A5 Antikörper (Intracellular)

Produktnummer ABIN7043668

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SLC1A5 in WB, IHC und IF. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für SLC1A5 Antikörper (Intracellular) (ABIN7043668)

Target: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC1A5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-SLC1A5 Antikörper

Bindungsspezifität: AA 502-516, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to ASCT2 Transporter

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): Will not recognize ASCT2 from human samples.

Homologie: Rat - 14,15 amino acid residues identical

Produktmerkmale: Anti-ASCT2 (SLC1A5) Antibody (ABIN7043668, ABIN7044823 and ABIN7044824) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry analysis. It has been designed to recognize SLC1A5 from mouse and rat samples. It will not recognize human ASCT2.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KMPSSEPELIQVKNE, corresponding to amino acid residues 502-516 of mouse SLC1A5

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7236918)

Blocking Peptide: (ABIN7236918)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu SLC1A5

Target: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Andere Bezeichnung: SLC1A5

Hintergrund: Alanine/serine/cysteine transporter 2, Neutral amino acid transporter B(0), ATB(0), ATBO, Sodium-dependent neutral amino acid transporter type 2, AAAT,ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways. It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.

Alternative names: ASCT2 (SLC1A5), Alanine/serine/cysteine transporter 2, Neutral amino acid transporter B(0), ATB(0), ATBO, Sodium-dependent neutral amino acid transporter type 2, AAAT

Gen-ID: 20514

NCBI Accession: NM_001145144

UniProt: P51912

Pathways: Dicarboxylic Acid Transport, Warburg Effekt