NALCN Antikörper (Extracellular)

Produktnummer ABIN7043658

Produktdetails anti-NALCN Antikörper

Target: NALCN (Sodium Leak Channel, Non-Selective (NALCN))

Bindungsspezifität: AA 902-914, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NALCN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)

Spezifität: Extracellular, S1-S2 domain III.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-NALCN/VGCNL1 (extracellular) Antibody (ABIN7043658 and ABIN7045249) is a highly specific antibody directed against an epitope of rat Sodium leak channel non-selective protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NALCN from mouse, rat, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SMMFESPFRRVMH, corresponding to amino acid residues 902-914 of rat NALCN

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7236886)

Blocking Peptide: (ABIN7236886)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu NALCN

Target: NALCN (Sodium Leak Channel, Non-Selective (NALCN))

Andere Bezeichnung: NALCN (NALCN Produkte)

Hintergrund: Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel,The existence of resting K+ conductance has been known for some time. Only recently has the Nalcn (sodium leak channel non-selective protein) been found responsible for the resting Na+ conductance1.Nalcn is a member of the NaV and CaV channel family which have four homologous repeats of six transmembrane domains. One structural difference is that Nalcn is less positively charged in the voltage sensing segment1. The protein forms voltage-independent non-inactivating channel which is permeable to Na+, K+ and Ca2+ ions1,2.Evidence suggests that Nalcn is a non-redundant and thus essential channel since knock-out mice display disrupted respiratory rhythm and die within 24 hours of birth. In addition, isolated neurons from these mice have decreased excitability1.The channel is extensively expressed in the central nervous system1, heart, pituitary and adrenal glands and pancreatic islet cells2,3.In pancreatic islets, Nalcn is activated by acetylcholine via its physical association with M3 muscarinic receptor, and potentiates insulin secretion induced by glucose uptake2-4.

Alternative names: NALCN/VGCNL1, Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel

Gen-ID: 266760

NCBI Accession: NM_052867

UniProt: Q6Q760