SIGMAR1 Antikörper (Intracellular)

Produktnummer ABIN7043628

Produktdetails anti-SIGMAR1 Antikörper

Target: SIGMAR1 (sigma Non-Opioid Intracellular Receptor 1 (SIGMAR1))

Bindungsspezifität: AA 113-126, Intracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIGMAR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Sigma-1 Receptor

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse - identical

Produktmerkmale: Anti-Sigma-1 Receptor Antibody (ABIN7043628, ABIN7044522 and ABIN7044523)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize SIGMAR1 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Güteklasse: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SRGHSGRYWAEISD, corresponding to amino acid residues 113-126 of human SIGMAR1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-IP006

Blocking Peptide: BLP-IP006

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu SIGMAR1

Target: SIGMAR1 (sigma Non-Opioid Intracellular Receptor 1 (SIGMAR1))

Andere Bezeichnung: SIGMAR1 (SIGMAR1 Produkte)

Hintergrund: SIGMAR1, OPRS1, Sigma non-opioid intracellular receptor 1, SIG-1R, Aging-associated gene 8 protein, SR31747-binding protein, SRBP,Sigma receptors were first identified as a subtype of opioid receptors. The different members of the family are differentially distributed in various parts of the CNS and are responsible for a vast range of functions and behaviors1. Today, sigma receptors are mostly considered to be a separate receptor family with two main subtypes characterized.The sigma-1 receptor is encoded by a gene baring the same name and is a protein of 223 amino acids with two transmembrane domains and a typical endoplasmic reticulum localized signal near its short N terminus. Sigma receptors are mostly detected in the central nervous system with a high density in the spinal cord, pons, medulla oblongata, red nucleus, cerebellum and hippocampus.Sigma-1 is mainly localized on the mitochondrial associated endoplasmic reticulum membrane (MAM), forming a BiP chaperone structure with high sensitivity to the calcium ion. When activated by agonists such as cocaine or analgesics, sigma-1 receptors separate from BiP and translocate from MAM to other parts of the cell. Through regulation of inositol triphosphate (IP3) receptors, NMDA receptors, dopamine receptors and voltage-gated Ca2+ channels, sigma-1 receptors can influence TCA cycle, oxidative stress, mitochondrial function, neuron plasticity and the release of various neurotransmitters.The exact etiology and pathophysiology of Alzheimer's disease is not fully understood. A few hypotheses suggest the involvement of Sigma-1 in the progression of Alzheimer's disease. The suggested mechanisms include protection from Aβ toxicity, prevention of Tau protein hyperphosphorylation and the upregulation of acetylcholine release2.

Alternative names: Sigma-1 Receptor, SIGMAR1, OPRS1, Sigma non-opioid intracellular receptor 1, SIG-1R, Aging-associated gene 8 protein, SR31747-binding protein, SRBP

Gen-ID: 10280

NCBI Accession: NM_005866

UniProt: Q99720

Pathways: SARS-CoV-2 Protein Interaktom