Serotonin Receptor 4 Antikörper (2nd Extracellular Loop)

Produktnummer ABIN7887155

Der Kaninchen Polyklonal anti-Serotonin Receptor 4 Antikörper wird verwendet zum Nachweis von Serotonin Receptor 4 in Proben von Ratte. Er wurde validiert für WB, FACS, LCI und IC.

Kurzübersicht für Serotonin Receptor 4 Antikörper (2nd Extracellular Loop) (ABIN7887155)

Target: Serotonin Receptor 4 (HTR4)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Serotonin Receptor 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunochromatography (IC)

Güteklasse: KO Validated

Produktdetails anti-Serotonin Receptor 4 Antikörper

Bindungsspezifität: 2nd Extracellular Loop, AA 168-180

Verwendungszweck: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - 12,13 amino acid residues identical, human - 11

Produktmerkmale: Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (ABIN7043624, ABIN7045290 and ABIN7045291) is a highly specific antibody directed against an epitope of the rat Serotonin receptor 4. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications.  It has been designed to recognize 5HT4 receptor from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 mg per reaction

ICC: 1:100

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Serotonin Receptor 4

Target: Serotonin Receptor 4 (HTR4)

Andere Bezeichnung: HTR4

Hintergrund:

Synonyms: 5-Hydroxytryptamine receptor 4, Serotonin receptor 4

Description: 5-hydroxytryptamine receptor 4 (5-HT4 receptors) are a subclass among the large family of receptors mediating serotonin actions at central and peripheral targets. 5-HT4 receptor activation has been implicated in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.The 5-HT4 receptor, a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs), couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side3.Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington's disease, feeding disorders and stress-related disorders such as anxiety and depression5.

Gen-ID: 25324

NCBI Accession: NM_000870

UniProt: Q62758

Pathways: JAK-STAT Signalweg, cAMP Metabolic Process, S100 Proteine