PTH1R Antikörper (Extracellular)

Produktnummer ABIN7043429

Produktdetails anti-PTH1R Antikörper

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Bindungsspezifität: AA 241-255, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PTH1R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to Parathyroid Hormone 1 Receptor

Spezifität: 1st extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 14,15 amino acid residues identical

Produktmerkmale: Anti-PTH1R (extracellular) Antibody (ABIN7043429, ABIN7045112 and ABIN7045113)) is a highly specific antibody directed against an epitope of the rat Parathyroid hormone 1 receptor. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize PTH1R from human, mouse, and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DAVLYSGFTLDEAER, corresponding to amino acid residues 241-255 of rat PTH1R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:60

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IFC

Negative Control: BLP-PR051

Blocking Peptide: BLP-PR051

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu PTH1R

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Andere Bezeichnung: PTH1R (PTH1R Produkte)

Hintergrund: Parathyroid hormone 1 receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR, PTHR1,The parathyroid hormone receptor type 1 (PTHR1) is bound and activated by two peptide hormones: the parathyroid hormone (PTH) secreted by the parathyroid gland and by parathyroid hormone related peptide (PTHrP) which is produced locally in many tissues. Although these two peptides bind the same receptor, they exert different actions. PTH regulates the concentrations of Ca2+, phosphate ions and vitamin D in the blood, stimulates bone formation/resorption and is distributed to the kidneys where it regulates Ca2+ and phosphate transport, and is also detected in the liver1-3. PTHrP, on the other hand, plays a critical role early in development of bone, heart, mammary glands and other tissues, and is detected in the circulation only during lactation and secreted by some tumors1,2,4,5. PTHR1 is obviously localized to all areas and tissues where PTH and PTHrP are known to exert their effects (i.e. bone, kidney, blood, heart, etc.).PTHR1 belongs to the superfamily of G-protein coupled receptors. Like all members, PTHR1 has seven transmembrane spanning domains, an extracellular N-terminus and an intracellular C-terminal tail. Upon activation, the receptor couples to either Gs and Gq thereby activating adenylate cyclase and phospholipase C (PLC) respectively. In osteosarcoma cells, PTHR1 can also couple Gα2,6,7.PTH is being used today to treat osteoporosis, as a factor promoting bone formation. Mutations in PTHR1 are associated with Jansen's disease (hypercalcemia and dwarfism) and Eiken syndrome (cartilage tumors of bone)2,8-10.

Alternative names: PTH1R, Parathyroid hormone 1 receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR, PTHR1

Gen-ID: 56813

NCBI Accession: NM_000316

UniProt: P25961

Pathways: Regulation of Carbohydrate Metabolic Process