NETO1 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886400

Der Kaninchen Polyklonal anti-NETO1 Antikörper (ABIN7886400) detektiert spezifisch NETO1 in WB, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für NETO1 Antikörper (Extracellular, N-Term) (ABIN7886400)

Target: NETO1 (Neuropilin (NRP) and Tolloid (TLL)-Like 1 (NETO1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NETO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-NETO1 Antikörper

Bindungsspezifität: AA 24-37, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to NETO1

Kreuzreaktivität: Maus, Ratte

Homologie: Rat - identical, human - 12,14 amino acid residues identical

Produktmerkmale: Anti-NETO1 (extracellular) Antibody (ABIN7043376, ABIN7044384 and ABIN7044385) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize NETO1 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KKGTEKQITPETQK, corresponding to amino acid residues 24-37 of mouse NETO1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236106)

Blocking Peptide: (ABIN7236106)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu NETO1

Target: NETO1 (Neuropilin (NRP) and Tolloid (TLL)-Like 1 (NETO1))

Andere Bezeichnung: NETO1

Hintergrund:

Synonyms: Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, BCTL1, BTCL1

Description: NETO proteins are auxiliary subunits that play crucial roles in modulating the biophysical properties of Kainate receptors (KARs) and N-methyl-D-aspartate receptors (NMDARs). NETO proteins modulate these receptors both in vivo and in vitro. NETO1 is also responsible for the slow kinetics of postsynaptic KAR-mediated currents and regulate synaptic recruitment of Kainate receptors1.NETO1 is a single transmembrane domain protein containing two extracellular CUB domains of about 110 amino acids, a low-density lipoprotein receptor domain, and a cytoplasmic tail of almost 167 amino acids that includes a C-terminal TRV class I PDZ binding motif2.NETO1 and NETO2, are highly homologous, but have a different expression patterns and distinct functional effects. NETO1 subunit is expressed in high levels in the developing hippocampus and regulates axonal and presynaptic Kainate receptors at the CA3-CA1 region of the hippocampus. NETO1 deficiency resulted in impairs synaptogenesis and disrupts synchronization of the CA3-CA1 neuronal populations1,3. NETO1 is enriched in the post-synaptic density where it co-localizes with post-synaptic density-95 (PSD-95) and GluN12.

Gen-ID: 246317

NCBI Accession: NM_138999

UniProt: Q8R4I7

Pathways: Regulation of long-term Neuronal Synaptic Plasticity