Neuregulin 1 Antikörper (Extracellular)

Produktnummer ABIN7043353

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Neuregulin 1 in WB, IHC und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Neuregulin 1 Antikörper (Extracellular) (ABIN7043353)

Target: Neuregulin 1 (NRG1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Neuregulin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Neuregulin 1 Antikörper

Bindungsspezifität: AA 87-101, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Neuregulin-1

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): This antibody will recognize Neuregulin-1 Type I, Type II and Type IV. It will not recognize Neuregulin-1 Type III.

Homologie: Mouse,human - identical

Produktmerkmale: Anti-NRG1 (Neuregulin-1) (extracellular) Antibody (ABIN7043353, ABIN7044718 and ABIN7044719) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and could potentially detect the protein in living cells. It has been designed to recognize Neuregulin-1 from rat, human, and mouse samples. This antibody will recognize NRG1 Type I, Type II and Type IV. It will not recognize Neuregulin-1 Type III.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KIQKKPGKSELRINK, corresponding to amino acid residues 87-101 of human Neuregulin-1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7236026)

Blocking Peptide: (ABIN7236026)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Neuregulin 1

Target: Neuregulin 1 (NRG1)

Andere Bezeichnung: NRG1

Hintergrund: Glial growth factor, GGF, Heregulin, HRG, Sensory and motor neuron derived factor, SMDF, Neu differentiation factor, NDF,ErbB receptors are a family of tyrosine kinase receptors encoded by the erbB gene. These receptors commence signaling pathways such as the mitogen activated protein kinase (MAPK) and inositol 3-kinase (PI3K) pathways and have an important role in the control of cell growth and survival1. Neuregulins are a family of EGF-like factors that are produced from alternative RNA splicing of the same gene. Through their interaction with ErbB receptors, Neuregulins regulate normal developmental processes, plasticity, and oncogenesis2.Neuregulin-1 is one of the four known proteins of the Neuregulin family and can be found in many organs including the heart, breast, peripheral and central nervous system. Neuregulin-1 is encoded by a gene located on chromosome 8p13 which produces more than 15 different isoforms of the same protein. All isoforms contain a core EGF domain encoded by exon E130 but other elements of the protein vary. Most NRG1 isoforms are synthesized as transmembrane proproteins which undergo proteolytic cleavage processes3.Due to its important function in the central and peripheral nervous system as well as in other organs NRG1 has been implicated in a variety of pathologies. The NRG1 gene is suspected as a schizophrenia susceptibility gene. Mice with a mutated NRG1 exhibit elevated activity in open field and abnormal sensorimotor gating which correspond with schizophrenic like behavior in humans. These affects are reversed using anti-psychotic drugs4. In addition, Activation of NRG1 was found to improve cardiac survival and function in a rat and canine model of dilated and ischemic hearts5.

Alternative names: NRG1 (Neuregulin-1) , Glial growth factor, GGF, Heregulin, HRG, Sensory and motor neuron derived factor, SMDF, Neu differentiation factor, NDF

Gen-ID: 3084

NCBI Accession: NM_001159995

UniProt: Q02297

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation