MRS2 Antikörper (Mitochondrial Matrix)

Produktnummer ABIN7043335

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MRS2 in WB und IHC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für MRS2 Antikörper (Mitochondrial Matrix) (ABIN7043335)

Target: MRS2 (MRS2 Magnesium Homeostasis Factor Homolog (MRS2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MRS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-MRS2 Antikörper

Bindungsspezifität: AA 211-223, Mitochondrial Matrix

Verwendungszweck: A Rabbit Polyclonal Antibody to MRS2 Magnesium Transporter

Spezifität: N-terminus, mitochondrial matrix.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-MRS2 Antibody is directed against an epitope of the rat MRS2 magnesium transporter. Anti-MRS2 Antibody (ABIN7043335, ABIN7044851 and ABIN7044852) can be used in western blot analysis. It has been designed to recognize MRS2 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DPKHSSVDRSKLH, corresponding to amino acid residues 211-223 of rat MRS2 magnesium transporter

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7235967)

Blocking Peptide: (ABIN7235967)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu MRS2

Target: MRS2 (MRS2 Magnesium Homeostasis Factor Homolog (MRS2))

Andere Bezeichnung: MRS2

Hintergrund: MRS2 magnesium transporter, Magnesium transporter MRS2 homolog mitochondrial, MRS2-like protein, MRS2L,Magnesium is one of the most abundant cation in the cell. It is crucial for the proper functioning of various tissues such as the brain, muscle, bone and the nervous system. Currently there are only two types of Mg2+ transport systems which have had their complete structures discovered. MgtE and CorA are the prokaryotic homologues of the SLC41 and MRS2 magnesium transporters.In eukaryotes, MRS2 protein is expressed in mitochondrial inner membranes. MRS2 is required for normal mitochondrial magnesium homeostasis and for proper myelination in the central nervous system. Notably, the expression of MRS2 serves as a genetic hallmark for embryonic stem cells2.MRS2 is comprised of two adjacent transmembrane domains. The first domain ends with a gly-met-asn motif and additional secondary structures. A long N-terminal and a short C-terminal sequence are located in the inner side of the mitochondrial membrane leaving the short loop connecting the transmembrane domains protruding to its exterior.In addition to its Mg2+ permeability, MRS2 transporters somewhat transport Ni2+. However, they are not permeable to Ca2+, Mn2+ and Co2+. MRS2 transporters can be blocked by Cobalt(III)-hexaamine, an analog of the hydrated Mg2+ ion and their activity is regulated by matrix Mg2+ concentrations. Increase in Mg2+ concentration causes a shift of the current-voltage relationship to a more negative potential as expected from the reduction in driving force and significantly reduces the channels' open-time probability2.Several SNPs in the MRS2 gene are associated an increased risk of developing type 2 diabetes in women of African American and Hispanic American origin3.

Alternative names: MRS2, MRS2 magnesium transporter, Magnesium transporter MRS2 homolog mitochondrial, MRS2-like protein, MRS2L

Gen-ID: 79032

NCBI Accession: NM_001286264

UniProt: Q9ET09