LPAR3 Antikörper (Extracellular)

Produktnummer ABIN7043314

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LPAR3 in WB. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für LPAR3 Antikörper (Extracellular) (ABIN7043314)

Target: LPAR3 (Lysophosphatidic Acid Receptor 3 (LPAR3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LPAR3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-LPAR3 Antikörper

Bindungsspezifität: AA 16-29, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to LPA Receptor 3 (EDG7)

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 12,14 amino acid residues identical

Produktmerkmale: Anti-LPAR3 (EDG7) (extracellular) Antibody (ABIN7043314, ABIN7044570 and ABIN7044571) is a highly specific antibody directed against an epitope to the rat LPA3 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA3 receptor from mouse, rat, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSNTDTADEWTGTK, corresponding to amino acid residues 16-29 of rat LPA3 receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7235904)

Blocking Peptide: (ABIN7235904)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu LPAR3

Target: LPAR3 (Lysophosphatidic Acid Receptor 3 (LPAR3))

Andere Bezeichnung: LPAR3

Hintergrund: Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32,Lysophosphatidic acid (LPA) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptors have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family2. All six receptors are type I, rhodopsin-like G-protein coupled receptors (GPCRs) that differ in their tissue distribution and downstream signaling pathways3.GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side4.Expression of LPA3 receptor has been observed in human heart, testis, prostate, pancreas, lung, ovary, and brain5 and is most abundant in mouse testis, kidney, lung, small intestine, heart, stomach, spleen, brain, and thymus6.LPA3 receptor null mice are viable and grossly normal, but female nulls show a striking phenotype in the reproductive system7. However, despite the fact that LPA3 is expressed in the frontal cortex, hippocampus, and amygdala, no phenotypes related to LPA3 loss in the nervous system have been reported to date5.

Alternative names: LPAR3 (EDG7), Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32

Gen-ID: 66025

NCBI Accession: NM_012152

UniProt: Q8K5E0

Pathways: Regulation of Cell Size