Leukotriene B4 Receptor/BLT Antikörper (Extracellular) (Atto 488)

Produktnummer ABIN7043308

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Leukotriene B4 Receptor/BLT in FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Leukotriene B4 Receptor/BLT Antikörper (Extracellular) (Atto 488) (ABIN7043308)

Target: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leukotriene B4 Receptor/BLT Antikörper ist konjugiert mit Atto 488

Applikation: Flow Cytometry (FACS)

Produktdetails anti-Leukotriene B4 Receptor/BLT Antikörper (Atto 488)

Bindungsspezifität: AA 168-181, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to BLT1 Conjugated to the Fluorescent Dye ATTO-488

Spezifität: 2nd extracellular loop

Kreuzreaktivität (Details): Not recommended for use with rat and mouse samples.

Homologie: Monkey - identical

Produktmerkmale: Anti-Human BLT1 (extracellular) Antibody (ABIN7043307 and ABIN7044557) is a highly specific antibody directed against an extracellular epitope of the human Leukotriene B4 receptor 1. The antibody can be used for western blot and indirect flow cytometry applications. It has been designed to recognize LTB4R from human samples only. \nAnti-Human BLT1 (extracellular)-ATTO Fluor-488 Antibody (ABIN7043308) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Human BLT1 (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CFPRYPSEGHRAFH, corresponding to amino acid residues 168-181 of human LTB4R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Kommentare:

Negative Control: (ABIN7582041)

Blocking Peptide:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Leukotriene B4 Receptor/BLT

Target: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Andere Bezeichnung: LTB4R

Hintergrund: Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7,Leukotrienes (LTs) are lipid mediators prominently exerting proinflammatory responses. They are divided in two classes: cysteinyl LTs (cysLTs) which have a thioester linkage and LTB41. LTB4, a chemoattractant, plays a prominent role in the recruitment and activation of leukocytes2.LTB4 acts through two receptors which belong to the G-protein coupled receptor superfamily: BLT1 and BLT2. BLT1 displays high affinity for LTB4 and is highly expressed in leukocytes including granulocytes, macrophages, mast cells, dendritic cells and effector T cells3 and is expressed to much lower levels in the spleen, thymus, heart, skeletal muscle brain and liver2-4. BLT2 on the other hand is a low affinity receptor which does not display selectivity towards LTB45. Its expression is much more ubiquitous than that of BLT1. It is expressed in most human tissues, with the highest expression being in the spleen, liver, ovary, and leukocytes2,5-8. The two receptors could couple and activate different G-proteins, depending on the cell type and the cellular events activating the receptors2. However, activation of BLT1 usually correlates with increasing intracellular concentrations of Ca2+.The LTB4/BLT1 system is involved in many allergic reactions as well as asthma induced as an allergic response, where it plays a significant role in recruiting neutrophils and effector T cells into lungs as part of an inflammatory response induced by allergens1.

Alternative names: BLT1, Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7

Gen-ID: 1241

NCBI Accession: NM_001143919

UniProt: Q15722