LRRC26 Antikörper (C-Term, Intracellular)

Produktnummer ABIN7886900

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LRRC26 in WB. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für LRRC26 Antikörper (C-Term, Intracellular) (ABIN7886900)

Target: LRRC26 (Leucine Rich Repeat Containing 26 (LRRC26))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LRRC26 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-LRRC26 Antikörper

Bindungsspezifität: AA 301-314, C-Term, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to LRRC26

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): Won't recognize human LRRC26.

Homologie: Mouse - 12,14 amino acid residues identical

Produktmerkmale: Anti-LRRC26 Antibody (ABIN7043299, ABIN7044982 and ABIN7044983) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize LRRC26 from rat and mouse samples. It does not recognize the protein from human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RRTTVRHLIRRQPD, corresponding to amino acid residues 301-314 of rat LRRC26

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235852)

Blocking Peptide: (ABIN7235852)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu LRRC26

Target: LRRC26 (Leucine Rich Repeat Containing 26 (LRRC26))

Andere Bezeichnung: LRRC26

Hintergrund:

Synonyms: Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC

Description: BK (KCa1.1) channels are critically involved in diverse physiological processes in both electrically excitable and non-excitable cells.Leucine-rich repeat containing protein 26 (also known as BK channel auxiliary gamma subunit or LRRC26) is a single spanning transmembrane protein of ~37 kDa. LRRC26 protein contains five major leucine rich repeats in its extracellular domain which are all required for modulating BK channel activity. The single transmembrane segment of LRRC26 is required for co-assembly with the BKα subunit complex.When LRRC26 is acutely knocked down in LNCaP cells, the voltage-dependent activation of endogenous BK current shifts from a low to a high V1/2 value, whereas recombinant expression of LRRC26 cDNA in naive cells (such as PC3 prostate cancer cells and HEK 293 cells) normally lacking LRRC26 shifts BK channel activation from high to low V1/2 values, reproducing the channel behavior observed in LNCaP cells. This findings indicate that LRRC26 is sufficient to impart low voltage gating behavior to wild-type pore-forming, BKα subunits.LRRC26 lowers the energy barrier for activation of BK channels, and is able to stabilize channel opening in response to voltage, thereby maintaining channels in the open state to a much greater extent at any given membrane potential1.LRRC26 is expressed in cerebral artery myocytes where it is primarily plasma membrane localized and associated with BKα subunits. LRRC26 serves as an arterial myocyte BK channel auxiliary γ subunit that elevates voltage and apparent Ca2+ sensitivity to induce vasodilation2.

Gen-ID: 311803

NCBI Accession: NM_001013653

UniProt: Q6P7C4