HCN2 Antikörper (Intracellular)

Produktnummer ABIN7043279

Der Kaninchen Polyklonal anti-HCN2 Antikörper wird verwendet zum Nachweis von HCN2 in Proben von Human. Er wurde validiert für WB, IHC, IP, IF und IC.

Kurzübersicht für HCN2 Antikörper (Intracellular) (ABIN7043279)

Target: HCN2 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2 (HCN2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HCN2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunochromatography (IC)

Güteklasse: KO Validated

Produktdetails anti-HCN2 Antikörper

Bindungsspezifität: AA 147-161, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to HCN2 Channel

Spezifität: Intracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse - identical

Produktmerkmale: Anti-HCN2 Antibody (ABIN7043279, ABIN7044940 and ABIN7044941) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize HCN2 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EEAGPAGEPRGSQAS, corresponding to amino acid residues 147-161 of human HCN2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IP|IHC|ICC

Negative Control: (ABIN7235786)

Blocking Peptide: (ABIN7235786)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu HCN2

Target: HCN2 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2 (HCN2))

Andere Bezeichnung: HCN2

Hintergrund: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2, BCNG2, HAC-1,Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain having crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2The Ih currents are generated by the hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which comprises four homologous members, named HCN1-4. Each HCN subunit consist of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain to cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2The channels are closely related to each other and share homology of about 60 % . However, they are diverging from each other in their cytoplasmic N- and the C-terminus. The channels HCN1-4 mainly differ from each other with regard to their speed of activation and the extent by which they are modulated by cAMP. HCN1 is the fastest channel, followed by HCN2, HCN3 and HCN4.2,3HCN2 is the most abundant neuronal channel and is found almost ubiquitously in the brain.3

Alternative names: HCN2, Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2, BCNG2, HAC-1

Gen-ID: 114244

NCBI Accession: NM_001194

UniProt: Q9UL51