HOMER1 Antikörper (Intracellular)

Produktnummer ABIN7043273

Produktdetails anti-HOMER1 Antikörper

Target: HOMER1 (Homer Homolog 1 (HOMER1))

Bindungsspezifität: AA 293-296, Intracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HOMER1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Verwendungszweck: A Rabbit Polyclonal Antibody to Homer1

Spezifität: Intracellular

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): The antibody will not recognize Homer1 short isoform 3 (also known as Homer1a), but will recognize isoform 2 (also known as Homer1b).

Homologie: Mouse,human - identical

Produktmerkmale: Anti-Homer1 Antibody (ABIN7043273, ABIN7045168 and ABIN7045169) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Homer1 from human, mouse, and rat samples. The antibody will not recognize Homer1 short isoform 3 (also known as Homer1a), but will recognize isoform 2 (also known as Homer1b).

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KQEIDNARELQEQR, corresponding to amino acid residues 293-296 of rat Homer1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:500

Kommentare:

Negative Control: (ABIN7235762)

Blocking Peptide: (ABIN7235762)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu HOMER1

Target: HOMER1 (Homer Homolog 1 (HOMER1))

Andere Bezeichnung: HOMER1 (HOMER1 Produkte)

Hintergrund: Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47,Homer proteins regulate signal transduction, generation of synapses and receptor trafficking. The homer protein family is comprised of three members with each member alternatively spliced to long and short forms. All members of the Homer family are synaptic scaffolding proteins with an N-terminal EVH (Ena/VASP homology 1) and a C-terminal coiled-coil domain. Long forms of the protein contain both EVH1 and coiled-coil domains and are constitutively expressed, whereas the short forms contain only the EVH1 domain and are expressed in an activity dependent manner1.The long form of Homer1 assembles group 1 metabotropic glutamate receptors mGluR1 and mGluR5 in large macromolecular complexes at synapses as well as with components of the NMDA receptor complex at the neuronal cell membrane. In contrast, the short form of Homer1 lacks the ability of linking mGluR1/5 to synaptic proteins and functions as an endogenous negative modulator of the mGluR1/5-inositol 1,4,5-trisphosphate receptor signaling complex. Transgenic mice with overexpressed Homer1 in striatal medium spiny neurons demonstrate normal development of striatal structure and afferent/efferent connectivity. However, motor performance in behavioral tasks and striatal responses to the psychomotor stimulant amphetamine are significantly altered in these mice2.At concentrations

Alternative names: Homer1, Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47

Gen-ID: 29546

NCBI Accession: NM_004272

UniProt: Q9Z214

Pathways: Skeletal Muscle Fiber Development