GRM8 Antikörper (Extracellular)

Produktnummer ABIN7043256

Dieses Anti-GRM8-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GRM8 in WB, IHC und IF. Geeignet für Ratte.

Kurzübersicht für GRM8 Antikörper (Extracellular) (ABIN7043256)

Target: GRM8 (Glutamate Receptor, Metabotropic 8 (GRM8))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRM8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-GRM8 Antikörper

Bindungsspezifität: AA 365-377, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 8

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse,human - 12,13 amino acid residues identical

Produktmerkmale: Anti-mGluR8 (extracellular) Antibody is directed against an extracellular epitope of rat mGluR8. Anti-mGluR8 (extracellular) Antibody (ABIN7043256 and ABIN7044363) can be used in western blot and immunohistochemistry applications. It has been designed to recognize mGluR8 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ENFG(S)KLGSHGKR, corresponding to amino acid residues 365-377 of rat mGluR8

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7235709)

Blocking Peptide: (ABIN7235709)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GRM8

Target: GRM8 (Glutamate Receptor, Metabotropic 8 (GRM8))

Andere Bezeichnung: GRM8

Hintergrund: Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H,Metabotropic glutamate receptors (mGluRs), heptahelical transmembrane receptors coupled to G-proteins, contribute to the regulation of neuronal excitability and synaptic transmission1. Genes coding for eight mGluRs (denoted mGluR1-8) with different splice variants have been identified and classified into three groups based on sequence homology, pharmacology, and signaling pathways. Group I mGluRs (mGluR1 and mGluR5) are primarily located postsynaptically. Group II (mGluR2 and mGluR3) are present both postsynaptically and presynaptically outside the release site. Group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) couple to Gαi/o to inhibit adenylyl cyclase found in the presynaptic active zone2.The mGluR8 receptor is detected in the olfactory bulb, thalamus, pontine gray, cerebral cortex, hippocampus, cerebellum, and retina3. Electrophysiological studies suggest that mGluR8 functions as a presynaptic autoreceptor to regulate glutamate release from the lateral perforant path terminals in the mouse dentate gyrus4. It is suggested that mGluR8, like other group III mGluRs, controls glutamate release by inhibiting voltage-gated Ca2+ channels.Targeted deletion of the mGluR8 gene in mice produces subtle behavioral alterations such as reduced habituation to novelty, hyperactivity, context-dependent but not cue-dependent disruption of the freezing response in the fear conditioning test, and increased anxiety5.

Alternative names: mGluR8, Metabotropic glutamate receptor 8, GRM8, mGlu8, GPRC1H

Gen-ID: 60590

NCBI Accession: NM_000845

UniProt: P70579

Pathways: cAMP Metabolic Process, Synaptic Membrane