Metabotropic Glutamate Receptor 5 Antikörper (Extracellular)

Produktnummer ABIN7043252

Produktdetails anti-Metabotropic Glutamate Receptor 5 Antikörper

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Bindungsspezifität: AA 367-380, Extracellular

Reaktivität: Ratte

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser Metabotropic Glutamate Receptor 5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: A Guinea Pig Polyclonal Antibody to Metabotropic Glutamate Receptor 5

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 13,14 amino residues identical

Produktmerkmale: Guinea pig Anti-mGluR5 (extracellular) Antibody is directed against an epitope of rat mGluR5. Guinea pig Anti-mGluR5 (extracellular) Antibody , raised in guinea pigs, can be used in western blot analysis. It has been designed to recognize mGluR5 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-mGluR5 (extracellular) Antibody (ABIN7043250, ABIN7044332 and ABIN7044333) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7235697)

Blocking Peptide: (ABIN7235697)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Metabotropic Glutamate Receptor 5

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Andere Bezeichnung: GRM5 (GRM5 Produkte)

Hintergrund: Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E,Metabotropic glutamate G protein-coupled receptors are divided into three groups on the basis of their sequence similarity, pharmacology and signal transduction mechanisms. mGluR5 is a class I mGlu receptor and is widely expressed throughout the cortex, hippocampus, striatum, caudate nucleus and nucleus accumbens, areas involved in emotion, motivation and cognition. mGluR5 is primarily located post-synaptically and is coupled to the Gq/11 pathway.mGluR5 is comprised of an extracellular N-terminus, an intracellular C-terminus and seven transmembrane α helices (TM1-7). Native ligands can bind to the receptor's extracellular domain. This extracellular binding is consistent with the narrow allosteric pocket the receptor possesses due to the tight configuration of its helical bundle1. mGluR5 is physically coupled to NMDA receptors by various scaffolding proteins including PSD-95, Shank, and Homer, as well as via a direct interaction. In addition, mGluR5 is biochemically coupled to NMDA receptor function via PKC. As a result of these molecular and biochemical interactions, activation of mGluR5 results in enhanced functionality of the NMDA receptor.mGluR5 is also involved in dendrogenesis and spineogenesis. mGluR5-/- knockout mice exhibit elevated spine densities irrespective of spine type or location along their dendritic trees in comparison to mGluR5+/- animals2.Recent studies have shown that mGluR5 is a critical mediator and a potential therapeutic target for the treatment of numerous disorders of the central nervous system, including depression, anxiety, drug addiction, chronic pain, Fragile X syndrome, Parkinson's disease, and gastroesophageal reflux disease3.

Alternative names: mGluR5, Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E

Gen-ID: 24418

NCBI Accession: NM_000842

UniProt: P31424

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity