Metabotropic Glutamate Receptor 3 Antikörper (Extracellular)

Produktnummer ABIN7043248

Produktdetails anti-Metabotropic Glutamate Receptor 3 Antikörper

Target: Metabotropic Glutamate Receptor 3 (GRM3) (Glutamate Receptor, Metabotropic 3 (GRM3))

Bindungsspezifität: AA 24-38, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Metabotropic Glutamate Receptor 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 3

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human,monkey - 14,15 amino residues identical

Produktmerkmale: Anti-mGluR3 (extracellular) Antibody is directed against an extracellular epitope of rat mGluR3. The epitope is specific for mGluR3 and will not cross-react with the closely related mGluR2. Anti-mGluR3 (extracellular) Antibody (ABIN7043248, ABIN7044342 and ABIN7044343)) can be used in western blot, live cell imaging and immunohistochemistry applications. It has been designed to recognize mGluR3 from rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Güteklasse: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GDHNFMRREIKIEGD, corresponding to amino acids 24-38 of rat mGluR3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:600

Kommentare:

Cited Application: IFC

Negative Control: BLP-GC012

Blocking Peptide: BLP-GC012

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Metabotropic Glutamate Receptor 3

Target: Metabotropic Glutamate Receptor 3 (GRM3) (Glutamate Receptor, Metabotropic 3 (GRM3))

Andere Bezeichnung: GRM3 (GRM3 Produkte)

Hintergrund: Metabotropic glutamate receptor 3, GRM3, mGlu3, GPRC1C,L-glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group II of the metabotropic glutamate receptors includes the mGluR2 and mGluR3 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus that contains the glutamate binding site, and an intracellular C-terminus.1,2 mGluR2 and mGluR3 are coupled to Gi/G0 and hence inhibit cAMP formation following receptor activation.1,2mGluR3 is expressed in neurons and glia in many brain regions including the cerebral cortex, hippocampus and brain stem. mGluR3 expression is predominantly presynaptic consistent with its role as an inhibitory autoreceptor, that is, activation of mGluR3 by glutamate inhibits additional glutamate release from the same neurons.1,2Several lines of evidence suggest that mGluR3 is important for long term depression, glial function and neuroprotection. Some studies, though not all, have shown a genetic association of mGluR3 gene polymorphisms with psychosis and with schizophrenia-related phenotypes. Based in these findings, mGluR3 has been suggested as a therapeutic target for both psychosis and schizophrenia.3

Alternative names: mGluR3, Metabotropic glutamate receptor 3, GRM3, mGlu3, GPRC1C

Gen-ID: 24416

NCBI Accession: NM_000840

UniProt: P31422

Pathways: cAMP Metabolic Process, Synaptic Membrane