Metabotropic Glutamate Receptor 1 Antikörper (Extracellular) (Atto 488)

Produktnummer ABIN7043246

Der Kaninchen Polyklonal anti-Metabotropic Glutamate Receptor 1 Antikörper wird verwendet zum Nachweis von Metabotropic Glutamate Receptor 1 in Proben von Ratte. Er wurde validiert für IHC und IF.

Kurzübersicht für Metabotropic Glutamate Receptor 1 Antikörper (Extracellular) (Atto 488) (ABIN7043246)

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Metabotropic Glutamate Receptor 1 Antikörper ist konjugiert mit Atto 488

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Metabotropic Glutamate Receptor 1 Antikörper (Atto 488)

Bindungsspezifität: AA 501-516, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 1 Conjugated to the Fluorescent Dye ATTO-488

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human,dog - identical

Produktmerkmale: Anti-mGluR1 (extracellular) Antibody (ABIN7043245, ABIN7044330 and ABIN7044331) is a highly specific antibody directed against the extracellular N-terminus domain of rat mGluR1. The antibody can be used in western blot and immunocytochemistry applications, and recognizes mGluR1 in rat, mouse and human samples. \nAnti-mGluR1 (extracellular)-ATTO Fluor-488 Antibody (ABIN7043246) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-mGluR1 (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HEGVLNIDDYKIQMNK, corresponding to amino acids 501-516 of rat mGluR1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:20-1:60

Application Dilutions Western blot wb: N/A

Kommentare:

Negative Control: (ABIN7582041)

Blocking Peptide: (ABIN7235681)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Metabotropic Glutamate Receptor 1

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Andere Bezeichnung: GRM1

Hintergrund: Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13,L-Glutamate is the major excitatory neurotransmitter in the central nervous system. It operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus and an intracellular C-terminus. The N-terminus domain of Group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.mGluR1 and mGluR5 receptors signal through Gq/G11, that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. Additional downstream signaling pathways include the activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins have also been reported.mGluR1 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the hippocampus, cerebellum, olfactory bulb and thalamus.The mGluR1 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson's and Huntington's disease.

Alternative names: mGluR1, Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13

Gen-ID: 24414

NCBI Accession: NM_000838

UniProt: P23385