GRIK1 Antikörper (Extracellular)

Produktnummer ABIN7043237

Der Kaninchen Polyklonal Anti-GRIK1-Antikörper wurde für WB, IHC, IF, IC und LCI validiert. Er ist geeignet, GRIK1 in Proben von Ratte zu detektieren.

Kurzübersicht für GRIK1 Antikörper (Extracellular) (ABIN7043237)

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRIK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Produktdetails anti-GRIK1 Antikörper

Bindungsspezifität: Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Kainate Receptor GluK1

Spezifität: Extracellular, N-terminus

Homologie: Human,dog,monkey - 15,16 amino acid residues identical

Produktmerkmale: Anti-GRIK1 (GluK1) (extracellular) Antibody (ABIN7043237, ABIN7044334 and ABIN7044335) is directed against the extracellular N-terminus domain of the rat kainate receptor GluK1. The antibody can be used in western blot and immunofluorescence applications.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KASGEVSKHLYKVWKK, corresponding to amino acid residues 402-417 of rat kainate receptor GluK1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7235653)

Blocking Peptide: (ABIN7235653)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GRIK1

Target: GRIK1 (Glutamate Receptor, Ionotropic, Kainate 1 (GRIK1))

Andere Bezeichnung: GRIK1

Hintergrund: Glutamate receptor ionotropic kainate 1, Glutamate receptor 5, GluR5,L-Glutamate, the major excitatory neurotransmitter in the central nervous system (CNS), operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein-coupled receptors).The ligand-gated ion channel family consists of fifteen members that have been subdivided into three families based upon their pharmacological profile: the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-preferring receptors, the N-methyl-D-aspartate (NMDA)-preferring receptors, and the kainate-preferring receptors.The kainate receptor subfamily consists of five members that have been further subdivided into two classes based upon structural homology and functional characteristics. GluR5, GluR6, and GluR7receptor subunits share a high degree of homology and are able to form functional channels when expressed in heterologous systems. The KA-1 and KA-2 receptors are unable to form functional channels on their own, but when coexpressed with GluR5-7 receptor subunits, they form channels with high affinity for kainate.1,2Like AMPA receptors, the functional unit of endogenous kainate receptors is believed to be a tetramer, which can be either homomeric or heteromeric. Kainate receptors GluR5 and GluR6 (but not GluR7, KA-1, or KA-2) can undergo RNA editing, as in the AMPA receptor GluR2, a glutamine (Q) residue in the channel pore is edited to encode arginine (R) in the mature protein. Substitution of Q with R modulates the properties of the channel, producing channels with reduced single channel conductance and lower permeability to Ca2+.1,2GluR5 is highly expressed in dorsal root ganglion (DRG) neurons of the peripheral nervous system (PNS) as well as in several structures of the CNS including the amygdala, the hipoccampus, and Purkinje cells of the cerebellum.GluR5 has been implicated in the modulation of synaptic transmission and synaptic plasticity in both the CNS and PNS, but the exact physiological function of GluR5 is not well understood. Nevertheless, an involvement in persistent pain and some types of learning has been proposed.

Alternative names: GRIK1 (GluK1), Glutamate receptor ionotropic kainate 1, Glutamate receptor 5, GluR5

Gen-ID: 29559

NCBI Accession: NM_000830

UniProt: P22756

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity