GJD2 Antikörper (AA 170-182)

Produktnummer ABIN7043205

Produktdetails anti-GJD2 Antikörper

Target: GJD2 (Gap Junction Protein, delta 2, 36kDa (GJD2))

Bindungsspezifität: AA 170-182

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GJD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Verwendungszweck: A Rabbit Polyclonal Antibody to Connexin-36

Spezifität: Intracellular loop

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse,human - 12,13 amino acid residues identical

Produktmerkmale: Anti-Connexin-36 Antibody (ABIN7043205, ABIN7044087 and ABIN7044088)) is a highly specific antibody targeted against an epiotpe of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Cx36 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EVKELAPHPSGLR, corresponding to amino acid residues 170-182 of rat Connexin-36

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-CC209

Blocking Peptide: BLP-CC209

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GJD2

Target: GJD2 (Gap Junction Protein, delta 2, 36kDa (GJD2))

Andere Bezeichnung: GJD2 (GJD2 Produkte)

Hintergrund: CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9,The structural unit of the gap junctions is the connexon. Connexons are hexameric structures, formed by transcellular channels composed of connexins. Connexins (Cx) share a common structural basis, which is a four-transmembrane spanning protein, a cytoplasmic loop, two extracellular loops (E1-E2), one intracellular amino-(NT domain) and a carboxy-terminal (CT domain) region1.Connexin 36 (Cx36) is predominantly expressed in mammalian neuronal cells2. Cx36 is expressed in a variety of mammalian brain regions, but expression is particularly notable in the inferior olive, cerebellar cortex, olfactory bulbs, several brainstem nuclei, the CA3 subregion of the hippocampus and reticular thalamic nuclei3. Outside of the central nervous system, Cx36 also appears in motoneurons of adult rat spinal cord 4.Studies have shown that Cx36 is involved in some neural functions, including neuronal neurogenesis, neural fertility control and regulation of waking and sleep states, and also Cx36 plays an important role in N-methyl-D-aspartate-mediated neuronal death following neuronal injury5. A number of studies performed in humans and animals have confirmed the role of Cx36 in the generation and maintenance of seizure activity6.

Alternative names: Connexin-36, CX36, Gap junction delta2 protein, Gap junction alpha9 protein, GJD2, GJA9

Gen-ID: 50564

NCBI Accession: NM_020660

UniProt: O70610