GABARAP Antikörper (Intracellular)

Produktnummer ABIN7043179

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GABARAP in WB und IHC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für GABARAP Antikörper (Intracellular) (ABIN7043179)

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABARAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GABARAP Antikörper

Bindungsspezifität: AA 2-15, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to GABA Receptor Associated Protein

Spezifität: Intracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): Due to high homology with GABA receptor associated protein-like proteins the antibody will recognize:GABA receptor associated protein-like 1 (11/14 amino acid residues identical), (Accession Q0VGK0).GABA receptor associated protein-like 3 (11/14 amino acid residues identical), (Accession Q9BY60).May recognize GABA receptor associated protein-like 2 (8/14 amino acid residues identical), (Accession P60522).

Homologie: Mouse,human - identical

Produktmerkmale: Anti-GABARAP Antibody (ABIN7043179, ABIN7044532 and ABIN7044533) is a highly specific antibody directed against an extracellular epitope of the rat GABA receptor associated protein. The antibody can be used in western blot analysis. It has been designed to recognize GABARAP from human, mouse and rat samples. Due to high homology with GABA receptor associated protein-like proteins the antibody will recognize GABA receptor associated protein 1 and 3 and may recognize GABA receptor associated protein 2.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KFVYKEEHPFEKRR, corresponding to amino acid residues 2-15 of rat GABARAP

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:800

Kommentare:

Negative Control: (ABIN7235469)

Blocking Peptide: (ABIN7235469)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Andere Bezeichnung: GABARAP

Hintergrund: GABA receptor associated protein, GABARAPL,GABA(A) receptors are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABA associated protein (GABARAP) family interacts specifically with the γ1, γ2S and γ2L subunits of the GABA(A) receptor. GABARAP is expressed in a variety of tissues including heart, brain, placenta, liver and others. The genetic sequence encoding GABARAP is similar to microtubule-associated proteins 1A and 1B1.GABARAP has an asymmetric structure with a basic N-terminal region and an acidic C terminus. GABARAP has a compact fold consisting of four or five-stranded β-sheets with two α-helices on each side. The outer strands of the β-sheets are aligned anti-parallel to the inner strands and helices α3 and α4 are located on one side of the sheet. The presence of α1 and α2 is a unique feature of GABARAP and forms a hydrophilic patch suitable for protein-protein interface. GABARAP exists in a monomeric conformation at low protein concentration and a head-to-tail oligomeric conformation in high salt conditions.GABARAP is involved in intracellular trafficking and is an integral part of the intra-Golgi transport machinery. Thus it is detected in cellular compartments such as the Golgi apparatus and endoplasmic reticulum. GABARAP affects GABA(A) receptors' cell surface expression by increasing their intracellular trafficking. Disruption of cellular microtubules prevents the clustering of both GABARAP and GABA(A) receptors. GABARAP also interacts with other receptors such as the glycine receptor at the postsynaptic site2.

Alternative names: GABARAP, GABA receptor associated protein, GABARAPL

Gen-ID: 58974

NCBI Accession: NM_007278

UniProt: P60517

Pathways: Autophagie