GPR68 Antikörper (Extracellular) (FITC)

Produktnummer ABIN7043171

Produktdetails anti-GPR68 Antikörper (FITC)

Target: GPR68 (G Protein-Coupled Receptor 68 (GPR68))

Bindungsspezifität: AA 169-183, Extracellular

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR68 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR68 Conjugated to the Fluorescent Dye FITC

Spezifität: 2nd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse,human - 14,15 amino acid residues identical

Produktmerkmale: Anti-GPR68 (OGR1) (extracellular) Antibody (ABIN7043170, ABIN7044425 and ABIN7044426)) is a highly specific antibody directed against an epitope of the mouse ovarian cancer G-protein coupled receptor 1. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR68 from rat, mouse, and human samples. \nAnti-GPR68 (OGR1) (extracellular)-FITC Antibody (ABIN7043170, ABIN7044425 and ABIN7044426)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Kommentare:

Negative Control: RIC-001-F

Blocking Peptide: BLP-GR042

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR68

Target: GPR68 (G Protein-Coupled Receptor 68 (GPR68))

Andere Bezeichnung: GPR68 (GPR68 Produkte)

Hintergrund: Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor,All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54 % ) with the human receptor GPR42.GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.

Alternative names: GPR68/OGR1, Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor

Gen-ID: 238377

NCBI Accession: NM_001177676

UniProt: Q8BFQ3