GPR68 Antikörper (2nd Extracellular Loop)

Produktnummer ABIN7886429

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GPR68 in WB, FACS und LCI. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für GPR68 Antikörper (2nd Extracellular Loop) (ABIN7886429)

Target: GPR68 (G Protein-Coupled Receptor 68 (GPR68))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR68 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-GPR68 Antikörper

Bindungsspezifität: 2nd Extracellular Loop, AA 169-183

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR68 (OGR1)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse,human - 14,15 amino acid residues identical

Produktmerkmale: Anti-GPR68 (OGR1) (extracellular) Antibody (ABIN7043170, ABIN7044425 and ABIN7044426) is a highly specific antibody directed against an epitope of the mouse ovarian cancer G-protein coupled receptor 1. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR68 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IFC

Negative Control: (ABIN7235438)

Blocking Peptide: (ABIN7235438)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR68

Target: GPR68 (G Protein-Coupled Receptor 68 (GPR68))

Andere Bezeichnung: GPR68

Hintergrund:

Synonyms: Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor

Description: All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54 % ) with the human receptor GPR42.GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.

Gen-ID: 238377

NCBI Accession: NM_001177676

UniProt: Q8BFQ3