G Protein-Coupled Receptor 119 Antikörper (2nd Cytoplasmic Loop)

Produktnummer ABIN7886423

Der Kaninchen Polyklonal anti-G Protein-Coupled Receptor 119 Antikörper wird verwendet zum Nachweis von G Protein-Coupled Receptor 119 in Proben von Ratte. Er wurde validiert für WB.

Kurzübersicht für G Protein-Coupled Receptor 119 Antikörper (2nd Cytoplasmic Loop) (ABIN7886423)

Target: G Protein-Coupled Receptor 119 (GPR119)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser G Protein-Coupled Receptor 119 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-G Protein-Coupled Receptor 119 Antikörper

Bindungsspezifität: 2nd Cytoplasmic Loop, AA 186-200

Verwendungszweck: A Rabbit Polyclonal Antibody to Glucose-Dependent Insulinotropic Receptor (GPR119)

Kreuzreaktivität: Maus, Ratte

Homologie: human - 13,15 amino acid residues identical,Mouse - 14

Produktmerkmale: Anti-GPR119 Antibody is directed against an epitope of the rat Glucose-dependent insulinotropic receptor. Anti-GPR119 Antibody (ABIN7043147, ABIN7044419 and ABIN7044420) can be used in western blot analysis. It has been designed to recognize GPR119 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: CDMLK IASVHSQHIR, corresponding to amino acid residues 186-200 of rat GPR119

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235360)

Blocking Peptide: (ABIN7235360)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu G Protein-Coupled Receptor 119

Target: G Protein-Coupled Receptor 119 (GPR119)

Andere Bezeichnung: GPR119

Hintergrund:

Synonyms: Glucose-dependent insulinotropic receptor, GPCR2

Description: Glucose-dependent insulinotropic receptor (GPR119) is a rhodopsin-type member of the G protein-coupled receptor (GPCRs) superfamily. Like all members, it has seven transmembrane spanning domains, an extracellular N-terminus and intracellular C-terminus. Activation of the receptor leads to a rise in intracellular cAMP - suggesting a stimulation of adenylate cyclase, possibly by Gαs coupling. It is expressed mostly in enteroendocrine cells of the gastrointenstinal tract and b-cells of the pancreas. It was stipulated that GPR119 is a close relative to the cannabinoid receptors1,2.Phospholipids and fatty acid amides are endogenous ligands for GPR119. Notably, oleoethanolamide (OEA) seems to be an important agonist. Stimulation of GPR119 by its agonists causes b-cells to release insulin and enteroendocrine cells to release glucagon-like peptide-1 (GLP-1), this attribute makes GPR119 a strong therapeutic candidate for type-2 diabetes and obesity3,4. Furthermore, its ability to release GLP-1 upon stimulation by dietary fat has gained it the title "fat sensor"5 .

Gen-ID: 302813

NCBI Accession: NM_178471

UniProt: Q7TQN8

Pathways: Hormone Transport, Peptide Hormone Metabolism