G Protein-Coupled Receptor 119 Antikörper (AA 186-200)

Produktnummer ABIN7043147

Produktdetails anti-G Protein-Coupled Receptor 119 Antikörper

Target: G Protein-Coupled Receptor 119 (GPR119)

Bindungsspezifität: AA 186-200

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser G Protein-Coupled Receptor 119 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: A Rabbit Polyclonal Antibody to Glucose-Dependent Insulinotropic Receptor (GPR119)

Spezifität: 2nd intracellular loop

Kreuzreaktivität: Maus, Ratte

Homologie: human - 13,15 amino acid residues identical,Mouse - 14

Produktmerkmale: Anti-GPR119 Antibody is directed against an epitope of the rat Glucose-dependent insulinotropic receptor. Anti-GPR119 Antibody (ABIN7043147, ABIN7044419 and ABIN7044420) can be used in western blot analysis. It has been designed to recognize GPR119 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CDMLK IASVHSQHIR, corresponding to amino acid residues 186-200 of rat GPR119

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7235360)

Blocking Peptide: (ABIN7235360)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu G Protein-Coupled Receptor 119

Target: G Protein-Coupled Receptor 119 (GPR119)

Andere Bezeichnung: GPR119 (GPR119 Produkte)

Hintergrund: Glucose-dependent insulinotropic receptor, GPCR2,Glucose-dependent insulinotropic receptor (GPR119) is a rhodopsin-type member of the G protein-coupled receptor (GPCRs) superfamily. Like all members, it has seven transmembrane spanning domains, an extracellular N-terminus and intracellular C-terminus. Activation of the receptor leads to a rise in intracellular cAMP - suggesting a stimulation of adenylate cyclase, possibly by Gαs coupling. It is expressed mostly in enteroendocrine cells of the gastrointenstinal tract and b-cells of the pancreas. It was stipulated that GPR119 is a close relative to the cannabinoid receptors1,2.Phospholipids and fatty acid amides are endogenous ligands for GPR119. Notably, oleoethanolamide (OEA) seems to be an important agonist. Stimulation of GPR119 by its agonists causes b-cells to release insulin and enteroendocrine cells to release glucagon-like peptide-1 (GLP-1), this attribute makes GPR119 a strong therapeutic candidate for type-2 diabetes and obesity3,4. Furthermore, its ability to release GLP-1 upon stimulation by dietary fat has gained it the title "fat sensor"5 .

Alternative names: GPR119, Glucose-dependent insulinotropic receptor, GPCR2

Gen-ID: 302813

NCBI Accession: NM_178471

UniProt: Q7TQN8

Pathways: Hormone Transport, Peptide Hormone Metabolism