DRD4 Antikörper (3rd Intracellular Loop)

Produktnummer ABIN7886286

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch DRD4 in WB. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für DRD4 Antikörper (3rd Intracellular Loop) (ABIN7886286)

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DRD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-DRD4 Antikörper

Bindungsspezifität: 3rd Intracellular Loop, AA 215-228

Verwendungszweck: A Rabbit Polyclonal Antibody to D4 Dopamine Receptor

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 9,14 amino acid residues identical

Produktmerkmale: Anti-D4 Dopamine Receptor Antibody is directed against an epitope located at the 3rd intracellular loop of the rat D4 dopamine receptor. Anti-D4 Dopamine Receptor Antibody (ABIN7043110 and ABIN7044233) can be used in western blot analysis, and recognizes DRD4 from rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RRWEAARHTKLHSR, corresponding to amino acid residues 215-228 of rat DRD4 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235246)

Blocking Peptide: (ABIN7235246)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu DRD4

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Andere Bezeichnung: DRD4

Hintergrund:

Synonyms: DRD4, D4DR, D(2C) dopamine receptor

Description: The dopamine neurotransmitter belongs to the catecholamines and can therefore be further converted into adrenaline and noreadrenaline. Dopamine has various physiological roles, including learning and memory, motor output and endocrine regulation. It does so by binding and activating dopamine receptors which belong to the G-protein coupled receptor superfamily (GPCR)1.The D4 dopamine receptor (DRD4) belongs to the D2-like family as do D2 and D3 dopamine receptors. Like all GPCRs, it has seven transmembrane spanning membrane regions. Structure-wise, members of the family share high homology in the transmembrane domains and lower homology in the extracellular N-terminal and the intracellular C-terminal domains. Notably, the coding region of the 3rd intracellular loop of DRD4 is known to undergo extensive polymorphism2.Like many GPCRs, each dopamine receptor subtype can react with more than one G-protein giving rise to different signaling possibilities3. Whereas D2-like dopamine receptors are generally considered to couple to Gi, and therefore inhibit adenylyl cyclase, the signaling through DRD4 is complicated due to the polymorphisms in the 3rd intracellular loop. It seems that this region is important to G-coupling as different polyphormisms in the region influence the ability of DRD4 to couple to adenylyl cyclase and G-proteins4,5. D4 dopamine receptors also influence Ca2+ levels3,6. They could also interact with G-protein couple inwardly rectifying K+ channel to ultimately cause a decrease in the firing rate of neurons7.The distribution of DRD4 mostly includes the brain and is mainly found post-synaptically in dendritic shafts and spines of mammalian striatum8.

Gen-ID: 25432

NCBI Accession: NM_000797

UniProt: P30729

Pathways: cAMP Metabolic Process, Synaptic Membrane, Proton Transport, Photoperiodism, Negative Regulation of Transporter Activity